Development of a real-time PCR method for quantification of Prevotella histicola from the gut

被引：8
|
作者：
Balakrishnan, Baskar [1 ]
Luckey, David [1 ]
Marietta, Eric [2 ]
Karau, Melissa [3 ]
Patel, Robin [3 ]
Murray, Joseph [2 ]
Taneja, Veena [1 ]
机构：
[1] Mayo Clin, Dept Immunol, Rochester, MN 55905 USA
[2] Mayo Clin, Dept Gastroenterol, Rochester, MN 55905 USA
[3] Mayo Clin, Dept Clin Microbiol, Rochester, MN 55905 USA
来源：
ANAEROBE | 2017年 / 48卷
关键词：
Prevotella histicola; Real-time PCR quantification qPCR; Anaerobe; Gut microbiome; IDENTIFICATION; POPULATIONS; MICROBIOME; ARTHRITIS; BACTERIA; PRIMERS;
D O I：
10.1016/j.anaerobe.2017.06.019
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We designed species-specific primers and developed a qPCR method for enumerating P. histicola from intestinal samples. The two designed primer sets showed specificity for the target 16S rRNA gene of P. histicola. The absolute qPCR method was sensitive to quantify as few as 10(3) colony-forming units (CFU) in the gut. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码：37 / 41
页数：5
相关论文
共 50 条
  • [1] Development and evaluation of a method for the quantification of airborne Thermoactinomyces vulgaris by real-time PCR
    Betelli, Laetitia
    Duquenne, Philippe
    Grenouillet, Frederic
    Simon, Xavier
    Scherer, Emeline
    Gain, Evelyne
    Hartmann, Alain
    JOURNAL OF MICROBIOLOGICAL METHODS, 2013, 92 (01) : 25 - 32
  • [2] Real-time PCR, a method fit for detection and quantification of Erwinia amylovora
    Dreo, Tanja
    Pirc, Manca
    Ravnikar, Maja
    TREES-STRUCTURE AND FUNCTION, 2012, 26 (01): : 165 - 178
  • [3] Development of a real-time PCR assay for quantification of Citrobacter rodentium
    Sagaidak, Sofia
    Taibi, Amel
    Wen, Bijun
    Comelli, Elena M.
    JOURNAL OF MICROBIOLOGICAL METHODS, 2016, 126 : 76 - 77
  • [4] Development of a quantification method with real-time PCR for three Pratylenchus species causing damage to chrysanthemum in Japan
    Koyama, Yuki
    Toyota, Koki
    Miyamaru, Naoko
    Yoshida, Koichi
    Uesugi, Kenta
    NEMATOLOGY, 2016, 18 : 687 - 695
  • [5] Development of a LUX real-time PCR for the detection and quantification of human herpesvirus 7
    Bergallo, Massimiliano
    Costa, Cristina
    Terlizzi, Maria Elena
    Sidoti, Francesca
    Margio, Samuela
    Astegiano, Sara
    Ponti, Renata
    Cavallo, Rossana
    CANADIAN JOURNAL OF MICROBIOLOGY, 2009, 55 (03) : 319 - 325
  • [6] Conventional PCR Detection and Real-Time PCR Quantification of Reniform Nematodes
    Sayler, Ronald J.
    Walker, Courtney
    Goggin, Fiona
    Agudelo, Paula
    Kirkpatrick, Terrence
    PLANT DISEASE, 2012, 96 (12) : 1757 - 1762
  • [7] Real-Time PCR Quantification of Methanobrevibacter oralis in Periodontitis
    Bringuier, Amelie
    Khelaifia, Saber
    Richet, Herve
    Aboudharam, Gerard
    Drancourt, Michel
    JOURNAL OF CLINICAL MICROBIOLOGY, 2013, 51 (03) : 993 - 994
  • [8] Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR
    David M. Stevenson
    Paul J. Weimer
    Applied Microbiology and Biotechnology, 2007, 75 : 165 - 174
  • [9] Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR
    Stevenson, David M.
    Weimer, Paul J.
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2007, 75 (01) : 165 - 174
  • [10] Development of a novel DNA extraction method for identification and quantification of Mycobacterium avium subsp paratuberculosis from tissue samples by real-time PCR
    Park, Kun Taek
    Allen, Andrew J.
    Davis, William C.
    JOURNAL OF MICROBIOLOGICAL METHODS, 2014, 99 : 58 - 65
12345