Assuring the Biofunctionalization of Silicone Covalently Bonded to Rhamnolipids: Antibiofilm Activity and Biocompatibility

被引:5
作者
Dardouri, Maissa [1 ]
Bettencourt, Ana [1 ]
Martin, Victor [2 ,3 ]
Carvalho, Filomena A. [4 ]
Colaco, Bruno [5 ]
Gama, Adelina [5 ]
Ramstedt, Madeleine [6 ]
Santos, Nuno C. [4 ]
Fernandes, Maria H. [2 ,3 ]
Gomes, Pedro S. [2 ,3 ]
Ribeiro, Isabel A. C. [1 ]
机构
[1] Univ Lisbon, Fac Pharm, Res Inst Med iMed ULisboa, Ave Prof Gama Pinto, P-1649003 Lisbon, Portugal
[2] Univ Porto, Fac Dent Med, BoneLab Lab Bone Metab & Regenerat, Rua Dr Manuel Pereira da Silva, P-4200393 Porto, Portugal
[3] Univ Porto, LAQV REQUIMTE, P-4050453 Porto, Portugal
[4] Univ Lisbon, Fac Med, Inst Med Mol, Av Prof Egas Moniz, P-1649028 Lisbon, Portugal
[5] Univ Tras Os Montes & Alto Douro UTAD, Anim & Vet Res Ctr CECAV, Associate Lab Anim & Vet Sci AL4Anim, P-5000801 Vila Real, Portugal
[6] Umea Univ, Dept Chem, S-90187 Umea, Sweden
关键词
rhamnolipids; PDMS; flow chamber; translational rabbit model; antimicrobial; biocompatible; BIOFILM FORMATION; CELL-ADHESION; BONE-CEMENT; PREVENTION; SURFACES; AGENTS;
D O I
10.3390/pharmaceutics14091836
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Silicone-based medical devices composed of polydimethylsiloxane (PDMS) are widely used all over the human body (e.g., urinary stents and catheters, central venous catheters stents) with extreme clinical success. Nevertheless, their abiotic surfaces, being prone to microorganism colonization, are often involved in infection occurrence. Improving PDMS antimicrobial properties by surface functionalization with biosurfactants to prevent related infections has been the goal of different works, but studies that mimic the clinical use of these novel surfaces are missing. This work aims at the biofunctional assessment of PDMS functionalized with rhamnolipids (RLs), using translational tests that more closely mimic the clinical microenvironment. Rhamnolipids were covalently bonded to PDMS, and the obtained surfaces were characterized by contact angle modification assessment, ATR-FTIR analysis and atomic force microscopy imaging. Moreover, a parallel flow chamber was used to assess the Staphylococcus aureus antibiofilm activity of the obtained surfaces under dynamic conditions, and an in vitro characterization with human dermal fibroblast cells in both direct and indirect characterization assays, along with an in vivo subcutaneous implantation assay in the translational rabbit model, was performed. A 1.2 log reduction in S. aureus biofilm was observed after 24 h under flow dynamic conditions. Additionally, functionalized PDMS lessened cell adhesion upon direct contact, while supporting a cytocompatible profile, within an indirect assay. The adequacy of the biological response was further validated upon in vivo subcutaneous tissue implantation. An important step was taken towards biofunctional assessment of RLs-functionalized PDMS, reinforcing their suitability for medical device usage and infection prevention.
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页数:15
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