Analysis of caffeine and paraxanthine in human saliva with ultra-high-performance liquid chromatography for CYP1A2 phenotyping

被引：13
|
作者：
Jordan, Nan Yeun [1 ]
Mimpen, Jolet Y. [1 ]
van den Bogaard, Willie J. M. [1 ]
Flesch, Frits M. [1 ]
van de Meent, Michiel H. M. [1 ]
Torano, Javier Sastre [1 ]
机构：
[1] Univ Utrecht, Utrecht Inst Pharmaceut Sci, Biomol Anal, NL-3508 TB Utrecht, Netherlands
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2015年 / 995卷
关键词：
Caffeine; Paraxanthine; CYP1A2; Phenotyping; Saliva; UHPLC; IN-VIVO; PLASMA; THEOPHYLLINE; RATIO; DRUG;
D O I：
10.1016/j.jchromb.2015.05.020
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Cytochrome P450 1A2 (CYP1A2) plays an important role in drug metabolism. Caffeine (CAF) is converted into paraxanthine (PX) by this enzyme and is used as a xenobiotic substrate to determine the CYP1A2 phenotype in humans. A method for the quantification of CAF and PX in saliva was developed using liquid-liquid extraction with ethyl acetate and analysis with ultra-high-performance liquid chromatography. Peaks from CAF, PX and internal standard were resolved within 6 min. The method was validated from 0.05 to 5 mu g mL(-1) CAF and 0.025-2.5 mu g mL(-1) PX. Inter- and intra-day accuracies ranged from 91.2 to 107.2% with precisions <13.5%. The limits of detection were 0.16 and 0.63 ng mL(-1) for PX and CAF, respectively. PX/CAF concentration ratios from volunteers were 0.26-1.09 with mean ratios of 0.78 +/- 0.26 and 0.38 +/- 0.10 for regular and light/non-coffee drinkers, respectively. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：70 / 73
页数：4
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