Complete genome sequence of the Medicago microsymbiont Ensifer (Sinorhizobium) medicae strain WSM419

被引：80

作者：

Reeve, Wayne ^{[1
]}

Chain, Patrick ^{[2
,3
]}

O'Hara, Graham ^{[1
]}

Ardley, Julie ^{[1
]}

Nandesena, Kemanthi ^{[1
]}

Braeu, Lambert ^{[1
]}

Tiwari, Ravi ^{[1
]}

Malfatti, Stephanie ^{[2
,3
]}

Kiss, Hajnalka ^{[2
,3
]}

Lapidus, Alla ^{[2
]}

Copeland, Alex ^{[2
]}

Nolan, Matt ^{[2
]}

Land, Miriam ^{[2
,4
]}

Hauser, Loren ^{[2
,4
]}

Chang, Yun-Juan ^{[2
,4
]}

Ivanova, Natalia ^{[2
]}

Mavromatis, Konstantinos ^{[2
]}

Markowitz, Victor ^{[5
]}

Kyrpides, Nikos ^{[2
]}

Gollagher, Margaret ^{[6
]}

Yates, Ron ^{[1
,7
]}

Dilworth, Michael ^{[1
]}

Howieson, John ^{[1
,7
]}

机构：

[1] Murdoch Univ, Ctr Rhizobium Studies, Perth, WA, Australia

[2] DOE Joint Genome Inst, Walnut Creek, CA USA

[3] Lawrence Livermore Natl Lab, Livermore, CA USA

[4] Oak Ridge Natl Lab, Oak Ridge, TN USA

[5] Univ Calif Berkeley, Lawrence Berkeley Lab, Biol Data Management & Technol Ctr, Berkeley, CA 94720 USA

[6] Murdoch Univ, Inst Sustainabil & Technol Policy, Perth, WA, Australia

[7] Dept Agr & Food, S Perth, WA, Australia

来源：

STANDARDS IN GENOMIC SCIENCES | 2010年 / 2卷 / 01期

关键词：

microsymbiont; non-pathogenic; aerobic; Gram-negative rod; root-nodule bacteria; nitrogen fixation; Alphaproteobacteria; ADHAERENS CASIDA 1982; ACID TOLERANCE; ROOT-NODULE; SP-NOV; MELILOTI; GENE; TOOL; FIXATION; PROPOSAL; IDENTIFICATION;

D O I：

10.4056/sigs.43526

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Ensifer (Sinorhizobium) medicae is an effective nitrogen fixing microsymbiont of a diverse range of annual Medicago (medic) species. Strain WSM419 is an aerobic, motile, non-spore forming, Gram-negative rod isolated from a M. murex root nodule collected in Sardinia, Italy in 1981. WSM419 was manufactured commercially in Australia as an inoculant for annual medics during 1985 to 1993 due to its nitrogen fixation, saprophytic competence and acid tolerance properties. Here we describe the basic features of this organism, together with the complete genome sequence, and annotation. This is the first report of a complete genome sequence for a microsymbiont of the group of annual medic species adapted to acid soils. We reveal that its genome size is 6,817,576 bp encoding 6,518 protein-coding genes and 81 RNA only encoding genes. The genome contains a chromosome of size 3,781,904 bp and 3 plasmids of size 1,570,951 bp, 1,245,408 bp and 219,313 bp. The smallest plasmid is a feature unique to this medic microsymbiont.

引用

页码：77 / 86

页数：10

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