Ultrasound-Assisted Natural Deep Eutectic Solvent Extraction and Bioactivities of Flavonoids in Ampelopsis grossedentata Leaves

被引:33
|
作者
Zhen, Shiyu [1 ]
Chen, Si [1 ]
Geng, Sheng [1 ]
Zhang, Hao [1 ]
Chen, Yongsheng [2 ]
Liu, Benguo [1 ]
机构
[1] Henan Inst Sci & Technol, Sch Food Sci, Xinxiang 453003, Peoples R China
[2] Jinan Univ, Dept Food Sci & Engn, Guangzhou 510632, Peoples R China
基金
中国国家自然科学基金;
关键词
flavonoid; Ampelopsis grossedentata; natural deep eutectic solvent; ultrasound-assisted extraction; antioxidant activity; antiproliferative activity; GREEN EXTRACTION; ANTIOXIDANT; CANCER; OPTIMIZATION; POLYPHENOLS; STRESS; WATER; ACID; WOOD; DES;
D O I
10.3390/foods11050668
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
We performed ultrasound-assisted extraction coupled with natural deep eutectic solvents (NADES) to achieve the green and efficient preparation of flavonoid extract from Ampelopsis grossedentata leaves. We then evaluated its antioxidant and antiproliferative activities. A NADES consisting of choline chloride and glucose at a molar ratio of 4:1 with 20% water was determined to be the most suitable solvent. The optimal extraction conditions were: a liquid-to-solid ratio of 30 mL/g, an ultrasonication power of 490 W, and an ultrasonication time of 6.5 min. The actual flavonoid yield was 83.93%, which was close to the predicted yield. Further, 86.75% of the flavonoids were recovered by adding the same volume of phosphate buffer saline (100 mM, pH of 7.0) to the extract solution. Although the chemical antioxidant activities of the flavonoid extract were slightly inferior to those of dihydromyricetin, the flavonoid extract could still effectively inhibit the proliferation of human breast MDA-MB-231 cells by inducing cell apoptosis, retarding the cell cycle, changing the mitochondrial membrane potential and scavenging intracellular reactive oxygen species (ROS). The obtained results can provide a reference in the development of plant-derived functional foods.
引用
收藏
页数:15
相关论文
共 50 条
  • [41] Optimizing Ultrasound-Assisted Deep Eutectic Solvent Extraction of Bioactive Compounds from Chinese Wild Rice
    Zeng, Jia
    Dou, Yuqing
    Yan, Ning
    Li, Na
    Zhang, Huaibao
    Tan, Jia-Neng
    MOLECULES, 2019, 24 (15):
  • [42] Ultrasound-Assisted Extraction of Phenolic Compounds from Celtuce (Lactuca sativa var. augustana) Leaves Using Natural Deep Eutectic Solvents (NADES): Process Optimization and Extraction Mechanism Research
    Li, Shanshan
    Wang, Guangyu
    Zhao, Junjie
    Ou, Penghui
    Yao, Qingping
    Wang, Wei
    MOLECULES, 2024, 29 (10):
  • [43] Optimization and kinetics modeling of phenolics extraction from coffee silverskin in deep eutectic solvent using ultrasound-assisted extraction
    Taweekayujan, Supawat
    Somngam, Supitcha
    Pinnarat, Tanawan
    HELIYON, 2023, 9 (07)
  • [44] Utilization of natural deep eutectic solvents and ultrasound-assisted extraction as green extraction technique for the recovery of bioactive compounds from date palm (Phoenix dactylifera L.) seeds: An investigation into optimization of process parameters
    Airouyuwa, Jennifer Osamede
    Mostafa, Hussein
    Riaz, Asad
    Maqsood, Sajid
    ULTRASONICS SONOCHEMISTRY, 2022, 91
  • [45] Ultrasound-assisted Aqueous Two-Phase Extraction of Flavonoids from Scutellariae Radix and Evaluation of their Bioactivities In Vitro
    Ge, Zhenkai
    Zhao, Yongheng
    Ling, Xu
    Zhu, Chenpan
    Hao, Xincai
    CURRENT ANALYTICAL CHEMISTRY, 2024, 20 (02) : 133 - 145
  • [46] Ultrasound-Assisted Extraction of Polyphenols from Maritime Pine Residues with Deep Eutectic Solvents
    Duarte, Hugo
    Gomes, Valentim
    Aliano-Gonzalez, Maria Jose
    Faleiro, Leonor
    Romano, Anabela
    Medronho, Bruno
    FOODS, 2022, 11 (23)
  • [47] Ultrasound-assisted extraction of asiaticoside from Centella asiatica using betaine-based natural deep eutectic solvent
    Mohd Fuad F.
    Mohd Nadzir M.
    Industrial Crops and Products, 2023, 192
  • [48] Ultrasound-Assisted Extraction of Betalains from the Amaranthus Using Natural Deep Eutectic Solvents
    Gupta, Vivek
    Saraswat, Vaishali
    Alagarasan, Ganesh
    Nallamilli, Trivikram
    Ramireddy, Eswarayya
    K.s.m.s., Raghavarao
    PLANT FOODS FOR HUMAN NUTRITION, 2025, 80 (01)
  • [49] Five Significant Phenols from Phyllostachys glauca McClure Leaves Extracted Using Ultrasound-Assisted Deep Eutectic Solvent Extraction
    Hei, Jianqiang
    Wang, Jianjun
    Wang, Jin
    Zhang, Daoqi
    Song, Wenting
    Xun, Hang
    Guo, Xuefeng
    Yao, Xi
    SEPARATIONS, 2024, 11 (08)
  • [50] A Green Extraction Process for Polyphenols from Elderberry (Sambucus nigra) Flowers Using Deep Eutectic Solvent and Ultrasound-Assisted Pretreatment
    Kaltsa, Olga
    Lakka, Achillia
    Grigorakis, Spyros
    Karageorgou, Ioanna
    Batra, Georgia
    Bozinou, Eleni
    Lalas, Stavros
    Makris, Dimitris P.
    MOLECULES, 2020, 25 (04):