We performed ultrasound-assisted extraction coupled with natural deep eutectic solvents (NADES) to achieve the green and efficient preparation of flavonoid extract from Ampelopsis grossedentata leaves. We then evaluated its antioxidant and antiproliferative activities. A NADES consisting of choline chloride and glucose at a molar ratio of 4:1 with 20% water was determined to be the most suitable solvent. The optimal extraction conditions were: a liquid-to-solid ratio of 30 mL/g, an ultrasonication power of 490 W, and an ultrasonication time of 6.5 min. The actual flavonoid yield was 83.93%, which was close to the predicted yield. Further, 86.75% of the flavonoids were recovered by adding the same volume of phosphate buffer saline (100 mM, pH of 7.0) to the extract solution. Although the chemical antioxidant activities of the flavonoid extract were slightly inferior to those of dihydromyricetin, the flavonoid extract could still effectively inhibit the proliferation of human breast MDA-MB-231 cells by inducing cell apoptosis, retarding the cell cycle, changing the mitochondrial membrane potential and scavenging intracellular reactive oxygen species (ROS). The obtained results can provide a reference in the development of plant-derived functional foods.
机构:
Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China
Cent S Univ, Minist Educ, Key Lab Biomet, Changsha, Hunan, Peoples R ChinaCent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China
Li, Jiaokun
Wu, Chenchen
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Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R ChinaCent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China
Wu, Chenchen
Li, Fang
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Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R ChinaCent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China
Li, Fang
Yu, Runlan
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Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China
Cent S Univ, Minist Educ, Key Lab Biomet, Changsha, Hunan, Peoples R ChinaCent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China
Yu, Runlan
Wu, Xueling
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Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China
Cent S Univ, Minist Educ, Key Lab Biomet, Changsha, Hunan, Peoples R ChinaCent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China
Wu, Xueling
Shen, Li
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Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China
Cent S Univ, Minist Educ, Key Lab Biomet, Changsha, Hunan, Peoples R ChinaCent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China
Shen, Li
Liu, Yuandong
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Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China
Cent S Univ, Minist Educ, Key Lab Biomet, Changsha, Hunan, Peoples R ChinaCent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China
Liu, Yuandong
Zeng, Weimin
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Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China
Cent S Univ, Minist Educ, Key Lab Biomet, Changsha, Hunan, Peoples R China
CSIRO Mineral Resources, Clayton, AustraliaCent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China
机构:
Rangsit Univ, Coll Pharm, Drug & Herbal Prod Res & Dev Ctr, Pathum Thani, Thailand
Rangsit Univ, Coll Pharm, Dept Pharmacognosy, Pathum Thani, ThailandRangsit Univ, Coll Pharm, Drug & Herbal Prod Res & Dev Ctr, Pathum Thani, Thailand
Monton, Chaowalit
Wunnakup, Thaniya
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Rangsit Univ, Coll Pharm, Drug & Herbal Prod Res & Dev Ctr, Pathum Thani, ThailandRangsit Univ, Coll Pharm, Drug & Herbal Prod Res & Dev Ctr, Pathum Thani, Thailand
Wunnakup, Thaniya
Sueree, Lukman
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Hatyai Univ, Fac Sci & Technol, Dept Community Publ Hlth, Songkhla, ThailandRangsit Univ, Coll Pharm, Drug & Herbal Prod Res & Dev Ctr, Pathum Thani, Thailand
Sueree, Lukman
Suksaeree, Jirapornchai
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Rangsit Univ, Coll Pharm, Dept Pharmaceut Chem, Pathum Thani, ThailandRangsit Univ, Coll Pharm, Drug & Herbal Prod Res & Dev Ctr, Pathum Thani, Thailand
Suksaeree, Jirapornchai
Charoenchai, Laksana
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Rangsit Univ, Coll Pharm, Drug & Herbal Prod Res & Dev Ctr, Pathum Thani, ThailandRangsit Univ, Coll Pharm, Drug & Herbal Prod Res & Dev Ctr, Pathum Thani, Thailand
Charoenchai, Laksana
Jongcharoenkamol, Jira
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Naresuan Univ, Fac Pharmaceut Sci, Dept Pharmaceut Chem & Pharmacognosy, Phitsanulok, ThailandRangsit Univ, Coll Pharm, Drug & Herbal Prod Res & Dev Ctr, Pathum Thani, Thailand