Ultrasonic-assisted extraction (UAE) of polysaccharides from white button mushroom (Agaricus bisporus) was studied. The four parameters, ultrasonic power, extraction temperature, extraction time, and the ratio of water volume to raw material weight (W/M ratio), were optimized using the central composite design (CCD) with a quadratic regression model built by using response surface methodology (RSM). The optimal extraction conditions found were: ultrasonic power 230W, extraction temperature 70 degrees C, extraction time 62 min, and W/M ratio 30 ml/g, where the highest A. bisporus polysaccharides (ABPS) yield of 6.02% was achieved. From HPGPC analysis, the molecular weight of ABPS was 158 kDa. Chemical and spectroscopic studies illustrated ABPS was only composed of Glc with beta-type glycosidic bond. In addition, the antioxidative activity of ABPS was investigated by measuring its scavenging ability on DPPH and hydroxyl in vitro. The results indicated that ABPS has good antioxidant activity. (c) 2011 Elsevier Ltd. All rights reserved.
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[1]
[Anonymous], 2005, FOOD CARBOHYDRATES C, DOI [10.1201/9780203485286.ch2, DOI 10.1201/9780203485286]
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Tokyo Univ Marine Sci & Technol, Dept Food Sci & Technol, Minato Ku, Tokyo 1088477, JapanTokyo Univ Marine Sci & Technol, Dept Food Sci & Technol, Minato Ku, Tokyo 1088477, Japan
Bao, Huynh N. D.
;
Ochiai, Yoshihiro
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Univ Tokyo, Grad Sch Agr & Life Sci, Lab Aquat Mol Biol & Biotechnol, Bunkyo Ku, Tokyo 1138657, JapanTokyo Univ Marine Sci & Technol, Dept Food Sci & Technol, Minato Ku, Tokyo 1088477, Japan
Ochiai, Yoshihiro
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Ohshima, Toshiaki
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Tokyo Univ Marine Sci & Technol, Dept Food Sci & Technol, Minato Ku, Tokyo 1088477, JapanTokyo Univ Marine Sci & Technol, Dept Food Sci & Technol, Minato Ku, Tokyo 1088477, Japan
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YanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R ChinaYanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R China
Chen, XiaoPing
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Wang, WanXiang
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YanCheng 1th People Hosp, Dept Sci Educ, Yancheng City 224000, Jiangsu, Peoples R ChinaYanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R China
Wang, WanXiang
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Li, ShuiBing
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YanCheng 1th People Hosp, Dept Facil, Yancheng City 224000, Jiangsu, Peoples R ChinaYanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R China
Li, ShuiBing
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Xue, JinLing
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YanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R ChinaYanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R China
Xue, JinLing
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Fan, LiJun
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YanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R ChinaYanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R China
Fan, LiJun
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Sheng, ZhongJi
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Soochow Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, Suzhou 215006, Peoples R ChinaYanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R China
Sheng, ZhongJi
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Chen, YouGuo
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Soochow Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, Suzhou 215006, Peoples R ChinaYanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R China
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Tokyo Univ Marine Sci & Technol, Dept Food Sci & Technol, Minato Ku, Tokyo 1088477, JapanTokyo Univ Marine Sci & Technol, Dept Food Sci & Technol, Minato Ku, Tokyo 1088477, Japan
Bao, Huynh N. D.
;
Ochiai, Yoshihiro
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Univ Tokyo, Grad Sch Agr & Life Sci, Lab Aquat Mol Biol & Biotechnol, Bunkyo Ku, Tokyo 1138657, JapanTokyo Univ Marine Sci & Technol, Dept Food Sci & Technol, Minato Ku, Tokyo 1088477, Japan
Ochiai, Yoshihiro
;
Ohshima, Toshiaki
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Tokyo Univ Marine Sci & Technol, Dept Food Sci & Technol, Minato Ku, Tokyo 1088477, JapanTokyo Univ Marine Sci & Technol, Dept Food Sci & Technol, Minato Ku, Tokyo 1088477, Japan
机构:
YanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R ChinaYanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R China
Chen, XiaoPing
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Wang, WanXiang
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YanCheng 1th People Hosp, Dept Sci Educ, Yancheng City 224000, Jiangsu, Peoples R ChinaYanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R China
Wang, WanXiang
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Li, ShuiBing
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YanCheng 1th People Hosp, Dept Facil, Yancheng City 224000, Jiangsu, Peoples R ChinaYanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R China
Li, ShuiBing
;
Xue, JinLing
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YanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R ChinaYanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R China
Xue, JinLing
;
Fan, LiJun
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YanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R ChinaYanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R China
Fan, LiJun
;
Sheng, ZhongJi
论文数: 0引用数: 0
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机构:
Soochow Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, Suzhou 215006, Peoples R ChinaYanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R China
Sheng, ZhongJi
;
Chen, YouGuo
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Soochow Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, Suzhou 215006, Peoples R ChinaYanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R China