Effect of oral administration of Limosilactobacillus reuteri on intestinal barrier function and mucosal immunity of suckling piglets

被引:3
|
作者
Wang, Qi [1 ,2 ]
Wang, Jing [1 ,2 ]
Qi, Renli [1 ,2 ]
Qiu, Xiaoyu [1 ]
Sun, Qian [1 ]
Huang, Jinxiu [1 ,2 ]
Wang, Ruisheng [1 ,2 ]
机构
[1] Chongqing Acad Anim Sci, Chongqing 402460, Peoples R China
[2] Minist Agr, Chongqing Key Lab Pig Ind Sci, Key Lab Pig Ind Sci, Chongqing, Peoples R China
基金
中国国家自然科学基金;
关键词
Limosilactobacillus reuteri; piglets; intestinal barrier; mucosal immunity; ENTEROTOXIGENIC ESCHERICHIA-COLI; NECROTIZING ENTEROCOLITIS; PROBIOTICS; SEVERITY; TLR4;
D O I
10.1080/1828051X.2022.2048977
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The aim of present study was to evaluate the effect of Limosilactobacillus reuteri (L. reuteri) on the intestinal barrier function and mucosal immunity of piglets at different suckling phase. In this study, fifty-four neonatal piglets (Rongchang x Yorkshire) were orally administered with 1.0 x 10(10) CFU L. reuteri I5007 (dissolved in 2 ml 0.1% peptone) or 2 ml 0.1% peptone by gavage every day. The experiment had lasted for 20 days from the birth. Six piglets from each group were slaughtered at 10- and 20-day-old, respectively. Intestinal samples were collected for the evaluation of target genes by real-time PCR and western blot. The results showed that L. reuteri I5007 significantly increased the average daily gain, decreased the diarrhoea incidence, and improved the intestinal morphology of piglets (p < .05). Oral administration of L. reuteri I5007 also elevated the mRNA and protein level of jejunal and ileal Zonula Occludens-1, Claudin-1, and Occludin to enhance the intestinal mucosal barrier function. Additionally, L. reuteri I5007 treatment modulated toll-like receptors (TLRs) and porcine host defense peptides (HDPs) expression, such as increased the mRNA levels of ileal TLR9 and PBD2 at 10-day-old, jejunal TLR9 and ileal PG1-5 at 20-day-old, the protein level of ileal TLR2 at 20-day-old, and influenced the protein and mRNA level of ileal TLR4. These findings indicated that oral administration of L. reuteri I5007 could improve the intestinal mucosal barrier and enhance the intestinal innate immunity of piglets during suckling period.
引用
收藏
页码:612 / 623
页数:12
相关论文
共 50 条
  • [21] Food antigens, intestinal barrier and mucosal immunity
    Heyman, Martine
    CAHIERS DE NUTRITION ET DE DIETETIQUE, 2010, 45 (02): : 65 - 71
  • [22] Protective effect of Limosilactobacillus reuteri-fermented yogurt on mouse intestinal barrier injury induced by enterotoxigenic Escherichia coli
    Lu, Xi
    Zhang, Mingxin
    Ma, Yuzhe
    Li, Guohua
    Zhao, Xin
    Qian, Weisheng
    JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2023, 103 (15) : 7494 - 7505
  • [23] Modulatory Effects of Lactobacillus salivarius on Intestinal Mucosal Immunity of Piglets
    Jinhua Zhang
    Jun Deng
    Zhisheng Wang
    Chuanyan Che
    Yun-feng Li
    Qian Yang
    Current Microbiology, 2011, 62 : 1623 - 1631
  • [24] Postbiotics from Pichia kudriavzevii promote intestinal health performance through regulation of Limosilactobacillus reuteri in weaned piglets
    Zhang, Zhenting
    Guo, Qiujin
    Wang, Jing
    Tan, Hongyan
    Jin, Xuexia
    Fan, Yurong
    Liu, Jiali
    Zhao, Shumiao
    Zheng, Jinshui
    Peng, Nan
    FOOD & FUNCTION, 2023, 14 (08) : 3463 - 3474
  • [25] Modulatory Effects of Lactobacillus salivarius on Intestinal Mucosal Immunity of Piglets
    Zhang, Jinhua
    Deng, Jun
    Wang, Zhisheng
    Che, Chuanyan
    Li, Yun-feng
    Yang, Qian
    CURRENT MICROBIOLOGY, 2011, 62 (05) : 1623 - 1631
  • [26] Paradoxical effect of butyrate on intestinal mucosal barrier function in vitro
    Chen, W
    Peng, LY
    Holzman, IR
    Lin, J
    PEDIATRIC RESEARCH, 2003, 53 (04) : 172A - 172A
  • [27] Postnatal growth retardation impairs intestinal mucosal barrier in piglets
    Qi, Ming
    Tan, Bie
    Yin, Yulong
    Liao, Simeng
    Li, Jianjun
    Wang, Jing
    JOURNAL OF ANIMAL SCIENCE, 2019, 97 : 78 - 78
  • [28] Isoleucine Enhanced the Function of the Small Intestinal Mucosal Barrier in Weaned Piglets to Alleviate Rotavirus Infection
    Zhao, Rongkun
    Jiang, Changsheng
    Yuan, Yuchen
    Zhang, Shen
    Ghonaim, Ahmed H.
    Che, Chuanyan
    Li, Xiaojin
    Jin, Mengmeng
    Jin, Erhui
    Zeng, Xiangfang
    Li, Shenghe
    Ren, Man
    ANIMALS, 2024, 14 (21):
  • [29] Intestinal Inflammation and Mucosal Barrier Function
    Sanchez de Medina, Fermin
    Romero-Calvo, Isabel
    Mascaraque, Cristina
    Martinez-Augustin, Olga
    INFLAMMATORY BOWEL DISEASES, 2014, 20 (12) : 2394 - 2404
  • [30] Effects of oral monosodium glutamate administration on serum metabolomics of suckling piglets
    Wang, Zhaobin
    Zhang, Jun
    Wu, Pei
    Luo, Shiyu
    Li, Jianzhong
    Wang, Qiye
    Huang, Pengfei
    Li, Yali
    Ding, Xueqin
    Hou, Zhenping
    Wu, Duanqin
    Huang, Jing
    Tu, Qiang
    Yang, Huansheng
    JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION, 2020, 104 (01) : 269 - 279