A Hyperandrogenic Mouse Model to Study Polycystic Ovary Syndrome

被引:11
作者
Xue, Ping [1 ]
Wang, Zhiqiang [1 ]
Fu, Xiaomin [1 ,2 ]
Vvang, Junjiang [1 ,3 ]
Punchhi, Gopika [1 ]
Wolfe, Andrew [1 ,4 ]
Wu, Sheng [1 ,4 ,5 ]
机构
[1] Johns Hopkins Univ, Sch Med, Dept Pediat, Baltimore, MD 21218 USA
[2] Beijing Mil Gen Hosp, Dept Hlth, Beijing, Peoples R China
[3] Southern Med Univ, Guangzhou, Guangdong, Peoples R China
[4] Johns Hopkins Univ, Sch Med, Dept Mol & Cellular Physiol, Baltimore, MD 21218 USA
[5] Johns Hopkins Univ, Sch Med, Dept Gynecol & Obstet, Baltimore, MD 21218 USA
来源
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2018年 / 140期
基金
美国国家卫生研究院;
关键词
Developmental Biology; Issue; 140; Polycystic ovary syndrome; Dihydrotestosterone (DHT); Estrous cyclicity; Androgen; Puberty; female offspring; ANDROGEN; PREGNANCY; WOMEN; RAT;
D O I
10.3791/58379
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Hyperandrogenemia plays a critical role in reproductive and metabolic function in females and is the hallmark of polycystic ovary syndrome. Developing a lean PCOS-like mouse model that mimics women with PCOS is clinically meaningful. In this protocol, we describe such a model. By inserting a 4 mm length of DHT (dihydrotestosterone) crystal powder pellet (total length of pellet is 8 mm), and replacing it monthly, we are able to produce a PCOS-like mouse model with serum DHT levels 2 fold higher than mice not implanted with DHT (no-DHT). We observed reproductive and metabolic dysfunction without changing body weight and body composition. While exhibiting a high degree of infertility, a small subset of these PCOS-like female mice can get pregnant and their offspring show delayed puberty and increased testosterone as adults. This PCOS-like lean mouse model is a useful tool to study the pathophysiology of PCOS and the offspring from these PCOS-like dams.
引用
收藏
页数:10
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