Separation and purification of puerarin using β-cyclodextrin-coupled agarose gel media

被引：43

作者：

He, XL

Tan, TW

Xu, BZ

Janson, JC

机构：

[1] Univ Uppsala, Uppsala Biomed Ctr, Dept Surface Biotechnol, SE-75123 Uppsala, Sweden

[2] Beijing Univ Chem Technol, Dept Bioengn, Beijing 100029, Peoples R China

[3] Amersham Biosci R&D Dept, SE-75184 Uppsala, Sweden

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1022卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

stationary phases; LC; pharmaceutical analysis; puerarin; cyclodextrin; agarose; isoflavonoids;

D O I：

10.1016/j.chroma.2003.09.045

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The isoflavonoid puerarin, a well-known traditional Chinese drug, has been purified in one step from an extract of Radix puerariae (root of the plant Pueraria lobata) by adsorption chromatography on an epichlorohydrin polymerized P-cyclodextrin ligand coupled to brominated allyl-group substituted Sepharose HP. Acetic acid (10%) was used as the mobile phase and the optimum loading capacity was around 1.2 mg crude extract/ml packed gel. The purity of the collected puerarin was about 98% with a recovery of about 62%. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：77 / 82

页数：6

共 12 条

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[2] Determination of puerarin, daidzein and rutin in Pueraria lobata (Wild.) Ohwi by capillary electrophoresis with electrochemical detection [J].