Role of GxxxG Motifs in Transmembrane Domain Interactions

被引:143
作者
Teese, Mark G. [1 ]
Langosch, Dieter
机构
[1] Tech Univ Munich, Lehrstuhl Chem Biopolymere, D-85354 Freising Weihenstephan, Germany
关键词
HELIX-HELIX INTERACTIONS; AMYLOID PRECURSOR PROTEIN; RECEPTOR TYROSINE KINASE; POLYTOPIC MEMBRANE-PROTEINS; NOVO STRUCTURE PREDICTION; YEAST F1F0-ATP SYNTHASE; DIMERIZATION MOTIF; GLYCOPHORIN-A; STRUCTURAL BASIS; ATP SYNTHASE;
D O I
10.1021/acs.biochem.5b00495
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transmembrane (TM) helices of integral membrane proteins can facilitate strong and specific noncovalent protein protein interactions. Mutagenesis and structural analyses have revealed numerous examples in which the interaction between TM helices of single-pass membrane proteins is dependent on a GxxxG or (small)xxx(small) motif It is therefore tempting to use the presence of these simple motifs as an indicator of TM helix interactions. In this Current Topic review, we point out that these motifs are quite common, with more than 50% of single-pass TM domains containing a (small)xxx(small) motif. However, the actual interaction strength of motif-containing helices depends strongly on sequence context and membrane properties. In addition, recent studies have revealed several GxxxG-containing TM domains that interact via alternative interfaces involving hydrophobic, polar, aromatic, or even ionizable residues that do not form recognizable motifs. In multipass membrane proteins, GxxxG motifs can be important for protein folding, and not just oligomerization. Our current knowledge thus suggests that the presence of a GxxxG motif alone is a weak predictor of protein dimerization in the membrane.
引用
收藏
页码:5125 / 5135
页数:11
相关论文
共 163 条
[1]   Transmembrane Recognition of the Semaphorin Co-Receptors Neuropilin 1 and Plexin A1: Coarse-Grained Simulations [J].
Aci-Seche, Samia ;
Sawma, Paul ;
Hubert, Pierre ;
Sturgis, James N. ;
Bagnard, Dominique ;
Jacob, Laurent ;
Genest, Monique ;
Garnier, Norbert .
PLOS ONE, 2014, 9 (05)
[2]   Prediction of transmembrane helix orientation in polytopic membrane proteins [J].
Adamian, Larisa ;
Liang, Jie .
BMC STRUCTURAL BIOLOGY, 2006, 6
[3]   Eukaryotic CTR copper uptake transporters require two faces of the third transmembrane domain for helix packing, oligomerization, and function [J].
Aller, SG ;
Eng, ET ;
De Feo, CJ ;
Unger, VM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (51) :53435-53441
[4]   The dynamic disulphide relay of quiescin sulphydryl oxidase [J].
Alon, Assaf ;
Grossman, Iris ;
Gat, Yair ;
Kodali, Vamsi K. ;
DiMaio, Frank ;
Mehlman, Tevie ;
Haran, Gilad ;
Baker, David ;
Thorpe, Colin ;
Fass, Deborah .
NATURE, 2012, 488 (7411) :414-+
[5]   Experimental measurement of the strength of a Cα-H•••O bond in a lipid bilayer [J].
Arbely, E ;
Arkin, IT .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (17) :5362-5363
[6]   A trimerizing GxxxG motif is uniquely inserted in the severe acute respiratory syndrome (SARS) coronavirus spike protein transmembrane domain [J].
Arbely, Eyal ;
Granot, Zvi ;
Kass, Itamar ;
Orly, Joseph ;
Arkin, Isaiah T. .
BIOCHEMISTRY, 2006, 45 (38) :11349-11356
[7]   STRUCTURAL ORGANIZATION OF THE PENTAMERIC TRANSMEMBRANE ALPHA-HELICES OF PHOSPHOLAMBAN, A CARDIAC ION-CHANNEL [J].
ARKIN, IT ;
ADAMS, PD ;
MACKENZIE, KR ;
LEMMON, MA ;
BRUNGER, AT ;
ENGELMAN, DM .
EMBO JOURNAL, 1994, 13 (20) :4757-4764
[8]   The GxxxG motif of the transmembrane domain of subunit e is involved in the dimerization/oligomerization of the yeast ATP synthase complex in the mitochondrial membrane [J].
Arselin, G ;
Giraud, MF ;
Dautant, A ;
Vaillier, J ;
Brèthes, D ;
Coulary-Salin, B ;
Schaeffer, J ;
Velours, J .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2003, 270 (08) :1875-1884
[9]   Interaction landscape of membrane-protein complexes in Saccharomyces cerevisiae [J].
Babu, Mohan ;
Vlasblom, James ;
Pu, Shuye ;
Guo, Xinghua ;
Graham, Chris ;
Bean, Bjoern D. M. ;
Burston, Helen E. ;
Vizeacoumar, Franco J. ;
Snider, Jamie ;
Phanse, Sadhna ;
Fong, Vincent ;
Tam, Yuen Yi C. ;
Davey, Michael ;
Hnatshak, Olha ;
Bajaj, Navgeet ;
Chandran, Shamanta ;
Punna, Thanuja ;
Christopolous, Constantine ;
Wong, Victoria ;
Yu, Analyn ;
Zhong, Gouqing ;
Li, Joyce ;
Stagljar, Igor ;
Conibear, Elizabeth ;
Wodak, Shoshana J. ;
Emili, Andrew ;
Greenblatt, Jack F. .
NATURE, 2012, 489 (7417) :585-+
[10]   Janus model of the Na,K-ATPase β-subunit transmembrane domain:: Distinct faces mediate α/β assembly and β-β homo-oligomerization [J].
Barwe, Sonali P. ;
Kim, Sanguk ;
Rajasekaran, Sigrid A. ;
Bowie, James U. ;
Rajasekaran, Ayyappan K. .
JOURNAL OF MOLECULAR BIOLOGY, 2007, 365 (03) :706-714