Isolation of Phytochemicals from Salvia plebeia Using Countercurrent Chromatography Coupled with Reversed-phase HPLC

被引：1
作者：
Kil, Hyun Woo [1 ,2 ]
Rho, Taewoong [1 ,2 ]
Seo, Young Ju [1 ,2 ]
Yu, Aram [3 ]
Yoon, Kee Dong [1 ,2 ]
机构：
[1] Catholic Univ Korea, Coll Pharm, Bucheon Si, Kyeonggi Do, South Korea
[2] Catholic Univ Korea, Integrated Res Inst Pharmaceut Sci, Bucheon Si, Kyeonggi Do, South Korea
[3] Food Ind Promot Agcy Korea, Iksan Si 54576, Jeollabuk Do, South Korea
来源：
NATURAL PRODUCT SCIENCES | 2020年 / 26卷 / 03期
基金：
新加坡国家研究基金会;
关键词：
Countercurrent chromatography; Lamiaceae; Reversed-phase HPLC; Salvia plebeia;
D O I：
10.20307/nps.2020.26.3.236
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Salvia plebeia R. Br. is a plant which has been used as an edible crop and traditional medicine in Asian countries. In this study, HPLC-PDA analysis and countercurrent chromatography (CCC) coupled with reversed-phase (RP) HPLC method were applied to isolate ten isolates from 3.3 g of n-butanol soluble extract from hot-water extract of S. plebeia. The use of CCC enabled us to efficiently fractionate the starting material with less sample loss and facilitate the isolation of compounds from S. plebeia extract using RP-HPLC. The isolates were determined to be caffeic acid (1), 6-hydroxyluteolin 7-O-beta-D-glucoside (2), eudebeiolide B (3), (R)-rosmarinic acid (4), homoplantaginin (5), eudebeiolide D (6), plebeiolide C (7), salpleflavone (8), eupafolin (9) and hispidulin (10) based on the spectroscopic evidence.
引用
收藏
页码：236 / 243
页数：8
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