SUMO2/3 conjugation is an endogenous neuroprotective mechanism

被引：122

作者：

Datwyler, Anna Lena ^{[1
]}

Laettig-Tuennemann, Gisela ^{[1
]}

Yang, Wei ^{[2
]}

Paschen, Wulf ^{[1
,2
]}

Lee, Sabrina Lin Lin ^{[1
]}

Dirnagl, Ulrich ^{[1
,3
]}

Endres, Matthias ^{[1
,3
]}

Harms, Christoph ^{[1
,3
]}

机构：

[1] Charite, Ctr Stroke Res Berlin, Dept Expt Neurol, D-10115 Berlin, Germany

[2] Duke Univ, Med Ctr, Dept Anesthesiol, Multidisciplinary Neuroprotect Labs, Durham, NC 27710 USA

[3] Charite, Klin & Poliklin Neurol, Dept Expt Neurol, D-10115 Berlin, Germany

来源：

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM | 2011年 / 31卷 / 11期

基金：

美国国家卫生研究院;

关键词：

endogenous neuroprotection; lentiviral microRNA delivery; oxygen-glucose deprivation; SUMO conjugation; FOCAL CEREBRAL-ISCHEMIA; PROTEIN SUMOYLATION; MODIFIER CONJUGATION; HYPOTHERMIA; PATHWAY; GROWTH; CELLS;

D O I：

10.1038/jcbfm.2011.112

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Small ubiquitin-like modifier (SUMO) 2/3 but not SUMO1 conjugation is activated after transient cerebral ischemia. To investigate its function, we blocked neuronal SUMO2/3 translation through lentiviral microRNA delivery in primary cortical neurons. Viability was unaffected by SUMO2/3 silencing unless neurons were stressed by transient oxygen-glucose deprivation (OGD). Both 15 and 45 minutes of OGD were tolerated by control microRNA-expressing neurons but damaged >60% of neurons expressing SUMO2/3 microRNA. Damaging OGD (75 minutes) increased neuronal loss to 54% (control microRNA) and to 99% (SUMO2/3 microRNA). This suggests that activation of SUMO2/3 conjugation is an endogenous neuroprotective stress response. Journal of Cerebral Blood Flow & Metabolism (2011) 31, 2152-2159; doi:10.1038/jcbfm.2011.112; published online 24 August 2011

引用

页码：2152 / 2159

页数：8

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