CRISPR Craze Conquers the RNA World: Precise Manipulation of DNA and RNA Based on a Bacterial Defense System

被引：6

作者：

Rentmeister, Andrea ^{[1
]}

机构：

[1] Univ Munster, Inst Biochem & Cells In Mot Cluster Excellence EX, D-48149 Munster, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2015年 / 54卷 / 16期

关键词：

Cas9; CRISPR; RNA; RNA manipulation; RNA targeting; RECOGNITION; CLEAVAGE; PROBES;

D O I：

10.1002/anie.201500563

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

It cuts both ways: The CRISPR/Cas9 genome engineering system has been extended from DNA to RNA. The sequence-specific targeting and cleavage of RNA can be achieved with S. pyogenes Cas9-gRNA if an appropriate short single-stranded oligonucleotide, the "PAMmer", is added. This technique could lead to a general approach for sequence-specific RNA manipulation. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：4710 / 4712

页数：3

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