Reassortant H1N1 influenza virus vaccines protect pigs against pandemic H1N1 influenza virus and H1N2 swine influenza virus challenge

被引：3

作者：

Yang, Huanliang ^{[1
]}

Chen, Yan ^{[1
]}

Shi, Jianzhong ^{[1
]}

Guo, Jing ^{[1
]}

Xin, Xiaoguang ^{[1
]}

Zhang, Jian ^{[1
]}

Wang, Dayan ^{[2
]}

Shu, Yuelong ^{[2
]}

Qiao, Chuanling ^{[1
]}

Chen, Hualan ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Anim Influenza Lab, Minist Agr, State Key Lab Vet Biotechnol,Harbin Vet Res Inst, Harbin 150001, Peoples R China

[2] China CDC, Natl Inst Viral Dis Control & Prevent, Beijing 100052, Peoples R China

来源：

VETERINARY MICROBIOLOGY | 2011年 / 152卷 / 3-4期

关键词：

Influenza A virus; H1N1; Swine; Vaccine; Reassortant virus; A VIRUS; EXPERIMENTAL-INFECTION; EFFICACY; CHINA; TRANSMISSION; PATHOGENESIS; EMERGENCE; FARM;

D O I：

10.1016/j.vetmic.2011.04.032

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Influenza A (H1N1) virus has caused human influenza outbreaks in a worldwide pandemic since April 2009. Pigs have been found to be susceptible to this influenza virus under experimental and natural conditions, raising concern about their potential role in the pandemic spread of the virus. In this study, we generated a high-growth reassortant virus (SC/PR8) that contains the hemagglutinin (HA) and neuraminidase (NA) genes from a novel H1N1 isolate, A/Sichuan/1/2009 (SC/09), and six internal genes from A/Puerto Rico/8/34 (PR8) virus, by genetic reassortment. The immunogenicity and protective efficacy of this reassortant virus were evaluated at different doses in a challenge model using a homologous SC/09 or heterologous A/Swine/Guangdong/1/06(H1N2) virus (GD/06). Two doses of SC/PR8 virus vaccine elicited high-titer serum hemagglutination inhibiting (HI) antibodies specific for the 2009 H1N1 virus and conferred complete protection against challenge with either SC/09 or GD/06 virus, with reduced lung lesions and viral shedding in vaccine-inoculated animals compared with non-vaccinated control animals. These results indicated for the first time that a high-growth SC/PR8 reassortant H1N1 virus exhibits properties that are desirable to be a promising vaccine candidate for use in swine in the event of a pandemic H1N1 influenza. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：229 / 234

页数：6

共 35 条

[1] Replication, Pathogenesis and Transmission of Pandemic (H1N1) 2009 Virus in Non-Immune Pigs [J].

Brookes, Sharon M. ;

Nunez, Alejandro ;

Choudhury, Bhudipa ;

Matrosovich, Mikhail ;

Essen, Stephen C. ;

Clifford, Derek ;

Slomka, Marek J. ;

Kuntz-Simon, Gaelle ;

Garcon, Fanny ;

Nash, Bethany ;

Hanna, Amanda ;

Heegaard, Peter M. H. ;

Queguiner, Stephane ;

Chiapponi, Chiara ;

Bublot, Michel ;

Maldonado Garcia, Jaime ;

Gardner, Rebecca ;

Foni, Emanuela ;

Loeffen, Willie ;

Larsen, Lars ;

Van Reeth, Kristien ;

Banks, Jill ;

Irvine, Richard M. ;

Brown, Ian H. .

PLOS ONE, 2010, 5 (02)

[2] PATHOGENICITY OF A SWINE INFLUENZA H1N1 VIRUS ANTIGENICALLY DISTINGUISHABLE FROM CLASSICAL AND EUROPEAN STRAINS [J].

BROWN, IH ;

DONE, SH ;

SPENCER, YI ;

COOLEY, WA ;

HARRIS, PA ;

ALEXANDER, DJ .

VETERINARY RECORD, 1993, 132 (24) :598-602

[3] Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus [J].

Busquets, Nuria ;

Segales, Joaquim ;

Cordoba, Lorena ;

Mussa, Tufaria ;

Crisci, Elisa ;

Martin-Valls, Gerard E. ;

Simon-Grife, Meritxell ;

Perez-Simo, Marta ;

Perez-Maillo, Monica ;

Nunez, Jose I. ;

Abad, Francesc X. ;

Fraile, Lorenzo ;

Pina, Sonia ;

Majo, Natalia ;

Bensaid, Albert ;

Domingo, Mariano ;

Montoya, Maria .

VETERINARY RESEARCH, 2010, 41 (05)

[4]

Cameron MJ, 2009, J INFECT DEV COUNTR, V3, P157

[5] Clinical and Epidemiologic Characteristics of 3 Early Cases of Influenza A Pandemic (H1N1) 2009 Virus Infection, People's Republic of China, 2009 [J].

Cao Bin ;

Li Xingwang ;

Shu Yuelong ;

Jiang Nan ;

Chen Shijun ;

Xu Xiayuan ;

Wang Chan .

EMERGING INFECTIOUS DISEASES, 2009, 15 (09) :1418-1422

[6] Generation and evaluation of a high-growth reassortant H9N2 influenza A virus as a pandemic vaccine candidate [J].

Chen, HL ;

Subbarao, K ;

Swayne, D ;

Chen, Q ;

Lu, XH ;

Katz, J ;

Cox, N ;

Matsuoka, Y .

VACCINE, 2003, 21 (17-18) :1974-1979

[7] Antigenic and Genetic Characteristics of Swine-Origin 2009 A(H1N1) Influenza Viruses Circulating in Humans [J].

Garten, Rebecca J. ;

Davis, C. Todd ;

Russell, Colin A. ;

Shu, Bo ;

Lindstrom, Stephen ;

Balish, Amanda ;

Sessions, Wendy M. ;

Xu, Xiyan ;

Skepner, Eugene ;

Deyde, Varough ;

Okomo-Adhiambo, Margaret ;

Gubareva, Larisa ;

Barnes, John ;

Smith, Catherine B. ;

Emery, Shannon L. ;

Hillman, Michael J. ;

Rivailler, Pierre ;

Smagala, James ;

de Graaf, Miranda ;

Burke, David F. ;

Fouchier, Ron A. M. ;

Pappas, Claudia ;

Alpuche-Aranda, Celia M. ;

Lopez-Gatell, Hugo ;

Olivera, Hiram ;

Lopez, Irma ;

Myers, Christopher A. ;

Faix, Dennis ;

Blair, Patrick J. ;

Yu, Cindy ;

Keene, Kimberly M. ;

Dotson, P. David, Jr. ;

Boxrud, David ;

Sambol, Anthony R. ;

Abid, Syed H. ;

George, Kirsten St. ;

Bannerman, Tammy ;

Moore, Amanda L. ;

Stringer, David J. ;

Blevins, Patricia ;

Demmler-Harrison, Gail J. ;

Ginsberg, Michele ;

Kriner, Paula ;

Waterman, Steve ;

Smole, Sandra ;

Guevara, Hugo F. ;

Belongia, Edward A. ;

Clark, Patricia A. ;

Beatrice, Sara T. ;

Donis, Ruben .

SCIENCE, 2009, 325 (5937) :197-201

[8] Emergence of avian H1N1 influenza viruses in pigs in China [J].

Guan, Y ;

Shortridge, KF ;

Krauss, S ;

Li, PH ;

Kawaoka, Y ;

Webster, RG .

JOURNAL OF VIROLOGY, 1996, 70 (11) :8041-8046

[9]

Howden KJ, 2009, CAN VET J, V50, P1153

[10] In vitro and in vivo characterization of new swine-origin H1N1 influenza viruses [J].

Itoh, Yasushi ;

Shinya, Kyoko ;

Kiso, Maki ;

Watanabe, Tokiko ;

Sakoda, Yoshihiro ;

Hatta, Masato ;

Muramoto, Yukiko ;

Tamura, Daisuke ;

Sakai-Tagawa, Yuko ;

Noda, Takeshi ;

Sakabe, Saori ;

Imai, Masaki ;

Hatta, Yasuko ;

Watanabe, Shinji ;

Li, Chengjun ;

Yamada, Shinya ;

Fujii, Ken ;

Murakami, Shin ;

Imai, Hirotaka ;

Kakugawa, Satoshi ;

Ito, Mutsumi ;

Takano, Ryo ;

Iwatsuki-Horimoto, Kiyoko ;

Shimojima, Masayuki ;

Horimoto, Taisuke ;

Goto, Hideo ;

Takahashi, Kei ;

Makino, Akiko ;

Ishigaki, Hirohito ;

Nakayama, Misako ;

Okamatsu, Masatoshi ;

Takahashi, Kazuo ;

Warshauer, David ;

Shult, Peter A. ;

Saito, Reiko ;

Suzuki, Hiroshi ;

Furuta, Yousuke ;

Yamashita, Makoto ;

Mitamura, Keiko ;

Nakano, Kunio ;

Nakamura, Morio ;

Brockman-Schneider, Rebecca ;

Mitamura, Hiroshi ;

Yamazaki, Masahiko ;

Sugaya, Norio ;

Suresh, M. ;

Ozawa, Makoto ;

Neumann, Gabriele ;

Gern, James ;

Kida, Hiroshi .

NATURE, 2009, 460 (7258) :1021-U110

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