A dynamic study and modeling of the formation of polyhydroxyalkanoates combined with treatment of high strength wastewater

Production of biodegradable thermoplastics, polyhydroxyalkanoates (PHAs), from organic wastes may provide multiple benefits to environmental conservation efforts. In this study, microfiltration-coupled reactors were set up to study the dynamic behavior of a typical PHA-producing bacteria, Ralstonia eutropha, fed with a real acidic solution from starch acidogenesis in a continuous flow system. The major fermentation acids(butyric and acetic acids) were utilized by the PHA producers at a high conversion rate (> 95%) when the cells were suspended in a small volume of mineral solution (pH 7), but at a low conversion rate (< 10%) when the cells were suspended in an acidic solution (pH 4). The acids were consumed mainly for PHA synthesis and maintenance energy, which resulted in slow growth of PHA-producing cells and a washout of the cells in the continuous flow system. PHAs, however, were continuously synthesized and accumulated during the washout. A simple dynamic model is proposed for estimation of specific growth rates and PHA formation rates during the washout at two hydraulic retention times (HRT) or dilution rates. The net specific growth rate of PHA-producing cells was near zero at a hydraulic retention time (HRT) of around 30 h, but it increased to 0.01 h(-1) when the HRT was reduced to 18 h. The model also reveals that PHA was synthesized faster based on the active biomass (ABM) during the short HRT (10.3 mg PHA/g ABM.h) than during the long HRT (3.4 mg PHA/g ABM.h).