Comparative analysis of hydrogen-producing bacterial biofilms and granular sludge formed in continuous cultures of fermentative bacteria

被引：54

作者：

Chojnacka, Aleksandra ^{[1
]}

Blaszczyk, Mieczyslaw K. ^{[2
]}

Szczesny, Pawel ^{[1
,3
]}

Nowak, Kinga ^{[1
]}

Suminska, Martyna ^{[1
]}

Tomczyk-Zak, Karolina ^{[1
]}

Zielenkiewicz, Urszula ^{[1
]}

Sikora, Anna ^{[1
]}

机构：

[1] Inst Biochem & Biophys PAS, PL-02106 Warsaw, Poland

[2] Warsaw Univ Life Sci, Fac Agr & Biol, PL-02787 Warsaw, Poland

[3] Warsaw Univ, Fac Biol, PL-02096 Warsaw, Poland

来源：

BIORESOURCE TECHNOLOGY | 2011年 / 102卷 / 21期

关键词：

Biofilm; Granular sludge; 454-Pyrosequencing; Hydrogen fermentation; Molasses; BIOHYDROGEN PRODUCTION; MICROBIAL COMMUNITY; WASTE-WATER; BED; MOLASSES; BIOREACTORS;

D O I：

10.1016/j.biortech.2011.08.063

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

A system for biohydrogen production was developed based on long-term continuous cultures grown on sugar beet molasses in packed bed reactors. In two separate cultures, consortia of fermentative bacteria developed as biofilms on granitic stones. In one of the cultures, a granular sludge was also formed. Metagenomic analysis of the microbial communities by 454-pyrosequencing of amplified 16S rDNA fragments revealed that the overall biodiversity of the hydrogen-producing cultures was quite small. The stone biofilm from the culture without granular sludge was dominated by Clostridiaceae and heterolactic fermentation bacteria, mainly Leuconostocaeae. Representatives of the Leuconostocaeae and Enterobacteriaceae were dominant in both the granules and the stone biofilm formed in the granular sludge culture. The culture containing granular sludge produced hydrogen significantly more effectively than that containing only the stone biofilm: 5.43 vs. 2.8 mol H-2/mol sucrose from molasses, respectively. The speculations that lactic acid bacteria may favor hydrogen production are discussed. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：10057 / 10064

页数：8

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[21] Quantitative and qualitative β diversity measures lead to different insights into factors that structure microbial communities [J].