Identification of a gene encoding the last step of the L-rhamnose catabolic pathway in Aspergillus niger revealed the inducer of the pathway regulator

被引：13

作者：

Chroumpi, Tania ^{[1
,2
]}

Aguilar-Pontes, Maria Victoria ^{[1
,2
,5
]}

Peng, Mao ^{[1
,2
]}

Wang, Mei ^{[3
]}

Lipzen, Anna ^{[3
]}

Ng, Vivian ^{[3
]}

Grigoriev, Igor, V ^{[3
]}

Makela, Miia R. ^{[4
]}

de Vries, Ronald P. ^{[1
,2
]}

机构：

[1] Univ Utrecht, Westerdijk Fungal Biodivers Inst, Fungal Physiol, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands

[2] Univ Utrecht, Fungal Mol Physiol, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands

[3] DOE Joint Genome Inst, 2800 Mitchell Dr, Walnut Creek, CA 94598 USA

[4] Univ Helsinki, Dept Microbiol, POB 56,Viiltinkaari 9, Helsinki, Finland

[5] Concordia Univ, Ctr Struct & Funct Genom, 7141 Sherbrooke St West, Montreal, PQ H4B 1R6, Canada

来源：

MICROBIOLOGICAL RESEARCH | 2020年 / 234卷

基金：

芬兰科学院;

关键词：

L-rhamnose catabolic pathway; RhaR; Inducer; Pectinolytic enzymes; Gene regulation; ENZYMES; DEGRADATION;

D O I：

10.1016/j.micres.2020.126426

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In fungi, L-rhamnose (Rha) is converted via four enzymatic steps into pyruvate and L-lactaldehyde, which enter central carbon metabolism. In Aspergillus niger, only the genes involved in the first three steps of the Rha catabolic pathway have been identified and characterized, and the inducer of the pathway regulator RhaR remained unknown. In this study, we identified the gene (lkaA) involved in the conversion of L-2-keto-3-deoxyrhamnonate (L-KDR) into pyruvate and L-lactaldehyde, which is the last step of the Rha pathway. Deletion of lkaA resulted in impaired growth on L-rhamnose, and potentially in accumulation of L-KDR. Contrary to Delta lraA, Delta lrlA and Delta lrdA, the expression of the Rha-responsive genes that are under control of RhaR, were at the same levels in Delta lkaA and the reference strain, indicating the role of L-KDR as the inducer of the Rha pathway regulator.

引用

页数：10

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