Fabrication, Characterization, and Evaluation of Bionanocomposites Based on Natural Polymers and Antibiotics for Wound Healing Applications

被引：20

作者：

Radulescu, Marius ^{[1
]}

Holban, Alina Maria ^{[2
,3
,4
]}

Mogoanta, Laurentiu ^{[5
]}

Balseanu, Tudor-Adrian ^{[6
]}

Mogosanu, George Dan ^{[7
]}

Savu, Diana ^{[8
]}

Popescu, Roxana Cristina ^{[2
,8
]}

Fufa, Oana ^{[2
,9
]}

Grumezescu, Alexandru Mihai ^{[2
]}

Bezirtzoglou, Eugenia ^{[10
]}

Lazar, Veronica ^{[3
]}

Chifiriuc, Mariana Carmen ^{[3
,4
]}

机构：

[1] Univ Politehn Bucuresti, Fac Appl Chem & Mat Sci, Dept Inorgan Chem Phys Chem & Electrochem, 1-7 Polizu St, Bucharest 011061, Romania

[2] Univ Politehn Bucuresti, Fac Appl Chem & Mat Sci, Dept Sci & Engn Oxide Mat & Nanomat, 1-7 Polizu St, Bucharest 011061, Romania

[3] Univ Bucharest, Fac Biol, Dept Microbiol Immunol, 1-3 Portocalelor Lane,Sect 5, Bucharest 77206, Romania

[4] Univ Bucharest, Res Inst, Life Environm & Earth Sci, Spl Independentei 91-95, Bucharest 0500088, Romania

[5] Univ Med & Pharm Craiova, Res Ctr Microscop Morphol & Immunol, PetruRares St 5, Craiova 200349, Romania

[6] Univ Med & Pharm Craiova, Res Ctr Clin & Expt Med, 2 PetruRares St, Craiova 200349, Romania

[7] Univ Med & Pharm Craiova, Fac Pharm, Dept Pharmacognosy & Phytotherapy, PetruRares St 2, Craiova 200349, Romania

[8] Horia Hulubei Natl Inst Phys & Nucl Engn, Dept Life & Environm Phys, Bucharest 077125, Romania

[9] Natl Inst Laser Plasma & Radiat Phys, Lasers Dept, Bucharest 077125, Romania

[10] Democritus Univ Thrace, Fac Agr Dev, Dept Food Sci & Technol, Lab Microbiol Biotechnol & Hyg, Orestiada 68200, Greece

来源：

MOLECULES | 2016年 / 21卷 / 06期

关键词：

chitin; sodium alginate; drug delivery; cefepime; cefuroxime; L929 cell line; rats; anti-biofilm; TARGETED DRUG-DELIVERY; CHITIN-GLUCAN COMPLEX; FACILE PREPARATION; CHITOSAN; NANOPARTICLES; ANTIFUNGAL; NANOFIBERS; ANTIBACTERIAL; DERIVATIVES; EXTRACTION;

D O I：

10.3390/molecules21060761

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic. Compositional, structural, and morphological evaluations were performed by using the thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR) analytical techniques. In order to quantitatively and qualitatively evaluate the biocompatibility of the obtained composites, we performed the tetrazolium-salt (MTT) and agar diffusion in vitro assays on the L929 cell line. The evaluation of antimicrobial potential was evaluated by the viable cell count assay on strains belonging to two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus).

引用

页数：14

共 70 条

[1] Chitin and chitosan in selected biomedical applications [J].