GelMA Hydrogel Reinforced with 3D Printed PEGT/PBT Scaffolds for Supporting Epigenetically-Activated Human Bone Marrow Stromal Cells for Bone Repair

被引：10
作者：
Man, Kenny [1 ,2 ]
Alcala, Cesar [3 ]
Mekhileri, Naveen V. [3 ]
Lim, Khoon S. [3 ]
Jiang, Lin-Hua [4 ]
Woodfield, Tim B. F. [3 ]
Yang, Xuebin B. [1 ]
机构：
[1] Univ Leeds, Sch Dent, Biomat & Tissue Engn Grp, Leeds LS9 7TF, W Yorkshire, England
[2] Univ Birmingham, Sch Chem Engn, Edgbaston, Birmingham B15 2TT, W Midlands, England
[3] Univ Otago Christchurch, Dept Orthopaed Surg, CReaTE Grp, Christchurch 8011, New Zealand
[4] Univ Leeds, Sch Biomed Sci, Leeds LS2 9JT, W Yorkshire, England
来源：
JOURNAL OF FUNCTIONAL BIOMATERIALS | 2022年 / 13卷 / 02期
基金：
英国工程与自然科学研究理事会;
关键词：
HDAC inhibitor; MI192; epigenetics; hydrogel; GelMA; 3D printing; bone; tissue engineering; STEM-CELLS; OSTEOGENIC DIFFERENTIATION; IN-VITRO; FABRICATION; PROMOTES; GROWTH;
D O I：
10.3390/jfb13020041
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Epigenetic approaches using the histone deacetylase 2 and 3 inhibitor-MI192 have been reported to accelerate stem cells to form mineralised tissues. Gelatine methacryloyl (GelMA) hydrogels provide a favourable microenvironment to facilitate cell delivery and support tissue formation. However, their application for bone repair is limited due to their low mechanical strength. This study aimed to investigate a GelMA hydrogel reinforced with a 3D printed scaffold to support MI192-induced human bone marrow stromal cells (hBMSCs) for bone formation. Cell culture: The GelMA (5 wt%) hydrogel supported the proliferation of MI192-pre-treated hBMSCs. MI192-pre-treated hBMSCs within the GelMA in osteogenic culture significantly increased alkaline phosphatase activity (p <= 0.001) compared to control. Histology: The MI192-pre-treated group enhanced osteoblast-related extracellular matrix deposition and mineralisation (p <= 0.001) compared to control. Mechanical testing: GelMA hydrogels reinforced with 3D printed poly(ethylene glycol)-terephthalate/poly(butylene terephthalate) (PEGT/PBT) scaffolds exhibited a 1000-fold increase in the compressive modulus compared to the GelMA alone. MI192-pre-treated hBMSCs within the GelMA-PEGT/PBT constructs significantly enhanced extracellular matrix collagen production and mineralisation compared to control (p <= 0.001). These findings demonstrate that the GelMA-PEGT/PBT construct provides enhanced mechanical strength and facilitates the delivery of epigenetically-activated MSCs for bone augmentation strategies.
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页数：16
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