Monoclonal antibody based competitive ELISA for the detection of antibodies against epizootic haemorrhagic disease of deer virus

被引：19
作者：
Thevasagayam, JA
Woolhouse, TR
Mertens, PPC
Burroughs, JN
Anderson, J
机构：
[1] Institute for Animal Health, Pirbright, Woking
来源：
JOURNAL OF VIROLOGICAL METHODS | 1996年 / 57卷 / 02期
关键词：
epizootic haemorrhagic disease; monoclonal antibody; competitive ELISA; bluetongue;
D O I：
10.1016/0166-0934(95)01968-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A monoclonal antibody based competitive enzyme-linked immunosorbent assay (MC-ELISA) for the detection of antibodies against epizootic haemorrhagic disease of deer viruses (EHDV) is described. Test sera were competed with a monoclonal antibody against the VP7 protein of EHDV serotype 1. The assay was capable of detecting antibodies to all serotypes of EHDV but unlike the agar gel immunodiffusion (AGID) test, gave no cross-reactions with antibodies against bluetongue, Palyam or Tilligery viruses. The MC-ELISA was more sensitive than a polyclonal based ELISA reported previously (Thevasagayam et al., 1995b) and would be ideal for epidemiological surveys since it is suitable for the examination of antisera from all animal species without the need for individual anti-species enzyme conjugates.
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收藏
页码：117 / 126
页数：10
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