High-density lipoprotein antagonizes oxidized low-density lipoprotein by suppressing oxygen free-radical formation and preserving nitric oxide bioactivity

被引:44
作者
Lee, CM
Chien, CT
Chang, PY
Hsieh, MY
Jui, HY
Liau, CS
Hsu, SM
Lee, YT
机构
[1] Natl Taiwan Univ Hosp, Dept Internal Med, Coll Med, Taipei 100, Taiwan
[2] Natl Taiwan Univ Hosp, Dept Med Res, Coll Med, Taipei 100, Taiwan
[3] Natl Taiwan Univ Hosp, Dept Pathol, Coll Med, Taipei 100, Taiwan
关键词
high-density lipoprotein; oxidized low-density lipoprotein; nitric oxide; endothelial cells; free radicals;
D O I
10.1016/j.atherosclerosis.2005.03.029
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The antiatherogenic role of high-density lipoprotein (HDL) has been related to its ability to increase the activity of endothelial nitric oxide synthase (eNOS) and to protect low-density lipoprotein (LDL) against oxidative modification. The present study was aimed to determine whether and how HDL antagonizes oxidized LDL (oxLDL) that has been formed and accumulated in circulation. Pre-infusion of rats with HDL effectively prevented oxLDL-induced renal vascular constriction. Consistently, pre-incubation of human saphenous vein endothelial cells with HDL (100 mu g/ml) reversed the oxLDL-induced suppression of endothelium-dependent cyclic-GMP production in co-cultured smooth muscle cells. However, the changes of Akt phosphorylation and eNOS activity in endothelial cells in response to lipoprotein treatments under our assay condition were not significant. Intriguingly, pretreatment of human umbilical vein endothelial cells with HDL (50 mu g/ml) for only 30 s effectively reduced the level of free radicals generated by oxLDL or H2O2. In kidneys of living rats, renal arterial infusion of oxLDL greatly enhanced ischemia/reperfusion-induced free radicals, which could be attenuated by HDL pretreatment. We conclude that HDL may antagonize oxLDL on endothelial function through an Akt-independent pathway in which HDL preserves nitric oxide bioactivity by attenuating oxLDL-triggered free radical generation. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:251 / 258
页数:8
相关论文
共 50 条
[21]   Nitric oxide inhibition of lipoxygenase-dependent liposome and low-density lipoprotein oxidation: Termination of radical chain propagation reactions and formation of nitrogen-containing oxidized lipid derivatives [J].
Rubbo, H ;
Parthasarathy, S ;
Barnes, S ;
Kirk, M ;
Kalyanaraman, B ;
Freeman, BA .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1995, 324 (01) :15-25
[22]   Apolipoprotein A1 and high-density lipoprotein limit low-density lipoprotein transcytosis by binding SR-B1 [J].
Fung, Karen Y. Y. ;
Ho, Tse Wing Winnie ;
Xu, Zizhen ;
Neculai, Dante ;
Beauchemin, Catherine A. A. ;
Lee, Warren L. ;
Fairn, Gregory D. .
JOURNAL OF LIPID RESEARCH, 2024, 65 (04)
[23]   Oxidized low-density lipoprotein-dependent endothelial arginase II activation contributes to impaired nitric oxide signaling [J].
Ryoo, Sungwoo ;
Lemmon, Christopher A. ;
Soucy, Kevin G. ;
Gupta, Gaurav ;
White, Anthony R. ;
Nyhan, Daniel ;
Shoukas, Artin ;
Romer, Lewis H. ;
Berkowitz, Dan E. .
CIRCULATION RESEARCH, 2006, 99 (09) :951-960
[24]   The role of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) in comparison with whole egg yolk for sperm cryopreservation in rhesus monkeys [J].
Dong, Qiao-Xiang ;
Rodenburg, Sarah E. ;
Hill, Dana ;
VandeVoort, Catherine A. .
ASIAN JOURNAL OF ANDROLOGY, 2011, 13 (03) :459-464
[25]   Formation of lipid-protein adducts in low-density lipoprotein by fluxes of peroxynitrite and its inhibition by nitric oxide [J].
Trostchansky, A ;
Batthyány, C ;
Botti, H ;
Radi, R ;
Denicola, A ;
Rubbo, H .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2001, 395 (02) :225-232
[26]   Validity of a semiquantitative food-frequency questionnaire in men and postmenopausal women with high concentrations of low-density lipoprotein and low concentrations of high-density lipoprotein cholesterol [J].
Ellsworth, NM ;
Mackey, SF ;
Jatulis, DE ;
Stefanick, ML .
AMERICAN JOURNAL OF CLINICAL NUTRITION, 1997, 65 (04) :1299-1299
[27]   Probucol Protects Endothelial Progenitor Cells Against Oxidized Low-Density Lipoprotein via Suppression of Reactive Oxygen Species Formation In Vivo [J].
Zhang, Qingbin ;
Chen, Liming ;
Si, Zhihua ;
Bu, Haoran ;
Narasimhulu, Chandrakala A. ;
Song, Xueling ;
Cui, Ming-Yu ;
Liu, Hang ;
Lu, Tiewei ;
He, Guanglong ;
Parthasarathy, Sampath ;
Cui, Lianqun ;
Liu, Zhenguo ;
Cui, Yuqi .
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2016, 39 (01) :89-101
[28]   Melatonin protects against oxidized low-density lipoprotein-induced inhibition of nitric oxide production in human umbilical artery [J].
Wakatsuki, A ;
Okatani, Y ;
Ikenoue, N ;
Shinohara, K ;
Watanabe, K ;
Fukaya, T .
JOURNAL OF PINEAL RESEARCH, 2001, 31 (03) :281-288
[29]   Pomegranate juice reduces oxidized low-density lipoprotein downregulation of endothelial nitric oxide synthase in human coronary endothelial cells [J].
de Nigris, Filomena ;
Williams-Ignarro, Sharon ;
Botti, Chiara ;
Sica, Vincenzo ;
Ignarro, Louis J. ;
Napoli, Claudio .
NITRIC OXIDE-BIOLOGY AND CHEMISTRY, 2006, 15 (03) :259-263
[30]   ASSAY OF LOW-DENSITY-LIPOPROTEIN SUSCEPTIBILITY TO OXIDATION BY MEANS OF THE FREE-RADICAL GENERATOR AAPH [J].
BIFFANTI, S ;
BONANOME, A ;
MAIORINO, M ;
URSINI, F ;
PAGNAN, A .
NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES, 1994, 4 (03) :137-141