Affinity maturation of antibodies requires integrity of the adult thymus

被引:11
作者
AbuAttieh, Mouhammed [1 ,2 ,3 ,4 ]
Bender, Diane [1 ,2 ,3 ]
Liu, Esther [1 ,2 ,3 ]
Wettstein, Peter [5 ]
Platt, Jeffrey L. [1 ,2 ,3 ]
Cascalho, Marilia [1 ,2 ,3 ]
机构
[1] Univ Michigan, Dept Surg, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Microbiol, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Dept Immunol, Ann Arbor, MI 48109 USA
[4] Charles Univ Prague, Sch Med, Dept Allergy & Clin Immunol, Hradec Kralove, Czech Republic
[5] Mayo Clin, Coll Med, Dept Surg, Rochester, MN USA
关键词
Affinity maturation; Antibody-secreting cells; Immunoglobulin; T-cell repertoire; Thymus; PLASMA-CELL; B-CELLS; HUMORAL IMMUNITY; THYMECTOMY; ANTIGEN; MICE; COMPARTMENT; ACTIVATION; ABLATION; RECEPTOR;
D O I
10.1002/eji.201141889
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The generation of B-cell responses to proteins requires a functional thymus to produce CD4+ T cells which helps in the activation and differentiation of B cells. Because the mature T-cell repertoire has abundant cells with the helper phenotype, one might predict that in mature individuals, the generation of B-cell memory would proceed independently of the thymus. Contrary to that prediction, we show here that the removal of the thymus after the establishment of the T-cell compartment or sham surgery without removal of the thymus impairs the affinity maturation of antibodies. Because removal or manipulation of the thymus did not decrease the frequency of mutation of the Ig variable heavy chain exons encoding antigen-specific antibodies, we conclude that the thymus controls affinity maturation of antibodies in the mature individual by facilitating the selection of B cells with high-affinity antibodies.
引用
收藏
页码:500 / 510
页数:11
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