Novel mechanisms in nutrient activation of the yeast Protein Kinase A pathway

被引：22

作者：

Thevelein, Johan M. ^{[1
,2
]}

Bonini, Beatriz Monge ^{[1
,2
]}

Castermans, Dries ^{[1
,2
]}

Haesendonckx, Steven ^{[1
,2
]}

Kriel, Johan ^{[1
,2
]}

Louwet, Wendy ^{[1
,2
]}

Thayumanavan, Palvannan ^{[1
,2
]}

Popova, Yulia ^{[1
,2
]}

Rubio-Texeira, Marta ^{[1
,2
]}

Schepers, Wim ^{[1
,2
]}

Vandormael, Patrick ^{[1
,2
]}

Van Zeebroeck, Griet ^{[1
,2
]}

Verhaert, Peter ^{[1
,2
,3
]}

Versele, Matthias ^{[1
,2
]}

Voordeckers, Karin ^{[1
,2
]}

机构：

[1] Katholieke Univ Leuven, Mol Cell Biol Lab, Inst Bot & Microbiol, Louvain, Belgium

[2] VIB, Dept Mol Microbiol, Louvain, Belgium

[3] Delft Univ Technol, Dept Biotechnol, Delft, Netherlands

来源：

ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA | 2008年 / 55卷 / 02期

关键词：

yeast; Protein Kinase A (PKA); activation of PKA;

D O I：

10.1556/AMicr.55.2008.2.1

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In yeast the Protein Kinase A (PKA) pathway can be activated by a variety of nutrients. Fermentable sugars, like glucose and sucrose, trigger a spike in the cAMP level, followed by activation of PKA and phosphorylation of target proteins causing a.o. mobilization of reserve carbohydrates, repression of stress-related genes and induction of growth-related genes. Glucose and sucrose are sensed by a G-protein coupled receptor system that activates adenylate cyclase and also activates a bypass pathway causing direct activation of PKA. Addition of other essential nutrients, like nitrogen sources or phosphate, to glucose-repressed nitrogen-or phosphate-starved cells, also triggers rapid activation of the PKA pathway. In these cases cAMP is not involved as a second messenger. Amino acids are sensed by the Gap1 transceptor, previously considered only as an amino acid transporter. Recent results indicate that the amino acid ligand has to induce a specific conformational change for signaling. The same amino acid binding site is involved in transport and signaling. Similar results have been obtained for Pho84 which acts as a transceptor for phosphate activation of the PKA pathway. Ammonium activation of the PKA pathway in nitrogen-starved cells is mediated mainly by the Mep2 transceptor, which belongs to a different class of transporter proteins. Hence, different types of sensing systems are involved in control of the yeast PKA pathway by nutrients.

引用

页码：75 / 89

页数：15

共 38 条

[31] Nutrient-induced signal transduction through the protein kinase A pathway and its role in the control of metabolism, stress resistance, and growth in yeast [J].