Inhibition of steroidogenesis in Leydig cells by exogenous nitric oxide occurs independently of steroidogenic acute regulatory protein (StAR) mRNA

被引：34

作者：

Dobashi, M ^{[1
]}

Fujisawa, M ^{[1
]}

Yamazaki, T ^{[1
]}

Okuda, Y ^{[1
]}

Kanzaki, M ^{[1
]}

Tatsumi, N ^{[1
]}

Tsuji, T ^{[1
]}

Okada, H ^{[1
]}

Kamidono, S ^{[1
]}

机构：

[1] Kobe Univ, Sch Med, Dept Urol, Chuo Ku, Kobe, Hyogo 6500017, Japan

来源：

ARCHIVES OF ANDROLOGY | 2001年 / 47卷 / 03期

关键词：

Leydig cells; nitric oxide; steroidogenic acute regulatory protein; testosterone;

D O I：

10.1080/014850101753145915

中图分类号：

R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

摘要：

Nitric oxide (NO) plays multiple roles in the reproductive system. The authors studied the effect of NO on LH-stimulated steroidogenesis in primary cultures of rat Leydig cells, particularly seeking a link between inhibition of steroidogenesis and changes in expression of steroidogenic acute regulatory protein (SLAR). Sodium nitroprusside (SNP), an NO generator, did not alter basal testosterone, but dose-dependently reduced testosterone production in the Leydig cells stimulated by LH (100 ng/mL) at 3 h after addition of SNP. Induction of StAR mRNA transcripts could be detected as early as I h after the addition of LH, but no effect was detected of SNP on LH induction of StAR mRNA. StAR, then, is not affected in the inhibition by NO of LH-stimulated steroidogenesis in Leydig cells.

引用

页码：203 / 209

页数：7

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