Inhibition of steroidogenesis in Leydig cells by exogenous nitric oxide occurs independently of steroidogenic acute regulatory protein (StAR) mRNA

被引：34

作者：

Dobashi, M ^{[1
]}

Fujisawa, M ^{[1
]}

Yamazaki, T ^{[1
]}

Okuda, Y ^{[1
]}

Kanzaki, M ^{[1
]}

Tatsumi, N ^{[1
]}

Tsuji, T ^{[1
]}

Okada, H ^{[1
]}

Kamidono, S ^{[1
]}

机构：

[1] Kobe Univ, Sch Med, Dept Urol, Chuo Ku, Kobe, Hyogo 6500017, Japan

来源：

ARCHIVES OF ANDROLOGY | 2001年 / 47卷 / 03期

关键词：

Leydig cells; nitric oxide; steroidogenic acute regulatory protein; testosterone;

D O I：

10.1080/014850101753145915

中图分类号：

R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

摘要：

Nitric oxide (NO) plays multiple roles in the reproductive system. The authors studied the effect of NO on LH-stimulated steroidogenesis in primary cultures of rat Leydig cells, particularly seeking a link between inhibition of steroidogenesis and changes in expression of steroidogenic acute regulatory protein (SLAR). Sodium nitroprusside (SNP), an NO generator, did not alter basal testosterone, but dose-dependently reduced testosterone production in the Leydig cells stimulated by LH (100 ng/mL) at 3 h after addition of SNP. Induction of StAR mRNA transcripts could be detected as early as I h after the addition of LH, but no effect was detected of SNP on LH induction of StAR mRNA. StAR, then, is not affected in the inhibition by NO of LH-stimulated steroidogenesis in Leydig cells.

引用

页码：203 / 209

页数：7

共 23 条

[1]

ADAMS ML, 1994, J PHARMACOL EXP THER, V269, P230

[2]

ADAMS ML, 1992, LIFE SCI, V50, P35

[3] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[4] Effect of nitric oxide on rat adrenal zona fasciculata steroidogenesis [J].

Cymeryng, CB ;

Dada, LA ;

Podestá, EJ .

JOURNAL OF ENDOCRINOLOGY, 1998, 158 (02) :197-203

[5] Nitric oxide inhibits Leydig cell steroidogenesis [J].

DelPunta, K ;

Charreau, EH ;

Pignataro, OP .

ENDOCRINOLOGY, 1996, 137 (12) :5337-5343

[6] ANALYSIS OF NITRATE, NITRITE, AND [N-15]-LABELED NITRATE IN BIOLOGICAL-FLUIDS [J].

GREEN, LC ;

WAGNER, DA ;

GLOGOWSKI, J ;

SKIPPER, PL ;

WISHNOK, JS ;

TANNENBAUM, SR .

ANALYTICAL BIOCHEMISTRY, 1982, 126 (01) :131-138

[7]

HELLSTROM WJG, 1994, FERTIL STERIL, V61, P1117

[8] NG-METHYL-L-ARGININE INHIBITS TUMOR NECROSIS FACTOR-INDUCED HYPOTENSION - IMPLICATIONS FOR THE INVOLVEMENT OF NITRIC-OXIDE [J].

KILBOURN, RG ;

GROSS, SS ;

JUBRAN, A ;

ADAMS, J ;

GRIFFITH, OW ;

LEVI, R ;

LODATO, RF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (09) :3629-3632

[9] Interferon-γ inhibits the steroidogenic acute regulatory protein messenger ribonucleic acid expression and protein levels in primary cultures of rat Leydig cells [J].

Lin, T ;

Hu, JX ;

Wang, DL ;

Stocco, DM .

ENDOCRINOLOGY, 1998, 139 (05) :2217-2222

[10] Interleukin-1 inhibits Leydig cell steroidogenesis without affecting steroidogenic acute regulatory protein messenger ribonucleic acid or protein levels [J].

Lin, T ;

Wang, D ;

Stocco, DM .

JOURNAL OF ENDOCRINOLOGY, 1998, 156 (03) :461-467

← 1 2 3 →