Type II toxin-antitoxin systems are unevenly distributed among Escherichia coli phylogroups

被引:36
作者
Fiedoruk, Krzysztof [1 ]
Daniluk, Tamara [1 ]
Swiecicka, Izabela [2 ]
Sciepuk, Malgorzata [1 ]
Leszczynska, Katarzyna [1 ]
机构
[1] Med Univ Bialystok, Dept Microbiol, PL-15222 Bialystok, Poland
[2] Univ Bialystok, Dept Microbiol, PL-15950 Bialystok, Poland
来源
MICROBIOLOGY-SGM | 2015年 / 161卷
关键词
BACTERIAL PERSISTENCE; PHYLOGENETIC GROUP; BIOFILM FORMATION; STRESS-RESPONSE; VIRULENCE; HOST; SUSCEPTIBILITY; PROKARYOTES; DIVERSITY; GENOME;
D O I
10.1099/mic.0.082883-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Type II toxin-antitoxin systems (TAs) are bicistronic operons ubiquitous in prokaryotic genomes, displaying multilevel association with cell physiology. Various possible functions have been assigned to TAs, ranging from beneficial for their hosts, such as a stress response, dormancy and protection against genomic parasites, to detrimental or useless functions, such as selfish alleles. As there is a link between several Escherichia coli features (e.g. virulence, lifestyle) and the phylogeny of this species, we hypothesized a similar association with TAs. Using PCR we studied the distribution of 15 chromosomal and plasmidic type II TA loci in 84 clinical E. coli isolates in relation to their main phylogenetic groups (A, B1, B2 and D). In addition, we performed in silico searching of these TA loci in 60 completely sequenced E. coli genomes deposited in GenBank. The highest number of TA loci per strain was observed in group A (mean 8.2, range 5-12) and the lowest in group B2 (mean 4.2, range 2-8). Moreover, significant differences in the prevalence of nine chromosomal TAs among E. coli phylogroups were noted. In conclusion, the presence of some chromosomal TAs in E. coli is phylogroup-related rather than a universal feature of the species. In addition, their limited collection in group B2 clearly distinguish it from the other E. coli phylogroups.
引用
收藏
页码:158 / 167
页数:10
相关论文
共 47 条
[1]   BLAST Ring Image Generator (BRIG): simple prokaryote genome comparisons [J].
Alikhan, Nabil-Fareed ;
Petty, Nicola K. ;
Ben Zakour, Nouri L. ;
Beatson, Scott A. .
BMC GENOMICS, 2011, 12
[2]  
[Anonymous], 2013, Standard Operating Procedure for PulseNet PFGE of Escherichia coli O157:H7, Escherichia coli non-O157 (STEC), Salmonella serotypes, Shigella sonnei and Shigella flexneri
[3]   Distribution of chromosome length variation in natural isolates of Escherichia coli [J].
Bergthorsson, U ;
Ochman, H .
MOLECULAR BIOLOGY AND EVOLUTION, 1998, 15 (01) :6-16
[4]   Escherichia coli phylogenetic groups are associated with site of infection and level of antibiotic resistance in community-acquired bacteraemia: a 10 year population-based study in Denmark [J].
Bukh, Annette S. ;
Schonheyder, Henrik C. ;
Emmersen, Jeppe M. G. ;
Sogaard, Mette ;
Bastholm, Soren ;
Roslev, Peter .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2009, 64 (01) :163-168
[5]   Escherichia coli phylogenetic group determination and its application in the identification of the major animal source of fecal contamination [J].
Carlos, Camila ;
Pires, Mathias M. ;
Stoppe, Nancy C. ;
Hachich, Elayse M. ;
Sato, Maria I. Z. ;
Gomes, Tania A. T. ;
Amaral, Luiz A. ;
Ottoboni, Laura M. M. .
BMC MICROBIOLOGY, 2010, 10
[6]   The evolution of the Escherichia coli phylogeny [J].
Chaudhuri, Roy R. ;
Henderson, Ian R. .
INFECTION GENETICS AND EVOLUTION, 2012, 12 (02) :214-226
[7]   Rapid and simple determination of the Escherichia coli phylogenetic group [J].
Clermont, O ;
Bonacorsi, S ;
Bingen, E .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (10) :4555-4558
[8]   Evidence for a human-specific Escherichia coli clone [J].
Clermont, Olivier ;
Lescat, Mathilde ;
O'Brien, Claire L. ;
Gordon, David M. ;
Tenaillon, Olivier ;
Denamur, Erick .
ENVIRONMENTAL MICROBIOLOGY, 2008, 10 (04) :1000-1006
[9]   Doc Toxin Is a Kinase That Inactivates Elongation Factor Tu [J].
Cruz, Jonathan W. ;
Rothenbacher, Francesca P. ;
Maehigashi, Tatsuya ;
Lane, William S. ;
Dunham, Christine M. ;
Woychik, Nancy A. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2014, 289 (11) :7788-7798
[10]   Chromosomal toxin-antitoxin systems may act as antiaddiction modules [J].
De Bast, Manuel Saavedra ;
Mine, Natacha ;
Van Melderen, Laurence .
JOURNAL OF BACTERIOLOGY, 2008, 190 (13) :4603-4609