Detection of antibodies to the nonstructural protein (NS1) of influenza A virus allows distinction between vaccinated and infected horses

被引：69

作者：

Ozaki, H

Sugiura, T

Sugita, S

Imagawa, H

Kida, H ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Vet Med, Dept Dis Control, Sapporo, Hokkaido 0600818, Japan

[2] Japan Racing Assoc, Div Mol Biol, Tochigi Lab Equine Res Inst, Tochigi 3290412, Japan

来源：

VETERINARY MICROBIOLOGY | 2001年 / 82卷 / 02期

关键词：

influenza; virus NS1; horse;

D O I：

10.1016/S0378-1135(01)00366-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Antibodies to the nonstructural protein (NS1) of A/equine/Miami/1/63 (H3N8) influenza virus were detected exclusively in the sera of mice experimentally infected with A/Aichi/2/68 (H3N2) and horses infected with A/equine/Kentucky/1/81 (H3N8) or A/equine/La Plata/1/93 (H3N8), but not in those of the animals immunized with the inactivated viruses, by enzyme-linked immunosorbent assay (ELISA) using a recombinant NS1 as antigen. The results indicate that the present method is useful for serological diagnosis to distinguish horses infected with equine H3 influenza viruses from those immunized with the inactivated vaccine. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：111 / 119

页数：9

共 19 条

[1] MECHANISM OF INTERFERENCE BY DEFECTIVE-INTERFERING PARTICLES OF INFLUENZA-VIRUS - DIFFERENTIAL REDUCTION OF INTRACELLULAR SYNTHESIS OF SPECIFIC POLYMERASE PROTEINS [J].