Optimization of a Method for Preparation and Repopulation of the Tracheal Matrix for Allogenic Transplantation

被引：7

作者：

Kiselevsky, M. V. ^{[1
]}

Anisimova, N. Yu ^{[1
]}

Lebedinskaya, O. V. ^{[1
]}

Polotskii, B. E. ^{[1
]}

Davydov, M. I. ^{[1
]}

机构：

[1] Russian Acad Med Sci, NN Blokhin Russian Oncol Res Ctr, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2011年 / 151卷 / 01期

关键词：

transplantation; trachea; mesenchymal stem cells; CRYOPRESERVATION;

D O I：

10.1007/s10517-011-1270-7

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

A method for decellularization of the trachea for subsequent repopulation with allogenic MSC was optimized. Tracheas from C57BL/6 mice were devitalized and repopulated with MSC from BALB/c mice. The tracheal matrix with devitalized mucosa and intact cartilaginous structure, fit for repopulation with allogenic MSC, was obtained by chemical treatment with NaClO4. This approach seemed to be promising for transplantation of allogenic trachea.

引用

页码：107 / 113

页数：7

共 13 条

[11] Hydrated xenogeneic decellularized tracheal matrix as a scaffold for tracheal reconstruction
Remlinger, Nathaniel T.
Czajka, Caitlin A.
Juhas, Mark E.
Vorp, David A.
Stolz, Donna B.
Badylak, Stephen F.
Gilbert, Sebastien
Gilbert, Thomas W.
[J]. BIOMATERIALS, 2010, 31 (13) : 3520 - 3526
[12] Tissue-engineered airway replacement
Sato, Toshihiko
Nakamura, Tatsuo
[J]. LANCET, 2008, 372 (9655) : 2003 - 2004
[13] Immune Evasion by Chimeric Trachea
Sykes, Megan
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 2010, 362 (02) : 172 - 174

← 1 2 →