Detection of low molecular weight toxins using an optical phase method of ellipsometry

被引：33

作者：

Nabok, A. ^{[1
]}

Tsargorodskaya, A.

Mustafa, M. K.

Szekacs, I. ^{[2
,3
]}

Starodub, N. F. ^{[4
]}

Szekacs, A. ^{[2
]}

机构：

[1] Sheffield Hallam Univ, Dept Engn & Math, Mat & Engn Res Inst, Sheffield S1 2NU, S Yorkshire, England

[2] Hungarian Acad Sci, Inst Plant Protect, H-1525 Budapest, Hungary

[3] Hungarian Acad Sci, Inst Expt Med, H-1083 Budapest, Hungary

[4] Natl Univ Life & Environm Sci Ukraine, UA-03041 Kiev, Ukraine

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2011年 / 154卷 / 02期

关键词：

Spectroscopic ellipsometry; Total Internal Reflection Ellipsometry; Direct immunoassay; Competitive immunoassay; Kinetics of immune reaction; INTERNAL-REFLECTION ELLIPSOMETRY; SURFACE; BIOSENSOR; INTERFEROMETER; SENSITIVITIES; REGISTRATION; PRINCIPLES; MYCOTOXIN; SENSORS;

D O I：

10.1016/j.snb.2010.02.005

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The method of total internal reflection ellipsometry (TIRE), which records simultaneously two parameters Delta and psi related to the amplitudes and phases of p- and s-components of polarised light, respectively, was exploited here for detection of mycotoxins (T-2 and zearalenone). The above mycotoxins in sub-ppb concentrations were detected using TIRE in both direct and competitive immunoassay. The study of immune reaction kinetics allowed the evaluation of adsorption/desorption rates and the association constant for zearalenone. The investigation of grain products samples revealed the presence of high concentrations of T-2 (>600 ng/ml) in mould products. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：232 / 237

页数：6

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