Minimally invasive molecular delivery into the brain using optical modulation of vascular permeability

被引：38

作者：

Choi, Myunghwan ^{[1
]}

Ku, Taeyun ^{[2
]}

Chong, Kyuha ^{[2
]}

Yoon, Jonghee ^{[1
]}

Choi, Chulhee ^{[1
,2
,3
,4
]}

机构：

[1] Korea Adv Inst Sci & Technol, Dept Bio & Brain Engn, Taejon 305701, South Korea

[2] Korea Adv Inst Sci & Technol, Grad Sch Med Sci & Engn, Taejon 305701, South Korea

[3] Korea Adv Inst Sci & Technol, KI BioCentury, Taejon 305701, South Korea

[4] Korea Adv Inst Sci & Technol, Opt BioImaging Ctr, Taejon 305701, South Korea

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2011年 / 108卷 / 22期

关键词：

femtosecond pulsed laser; laser tissue interaction; molecular delivery; permeability; IN-VIVO; FEMTOSECOND LASER; CRANIAL WINDOW; BARRIER; CELLS; DISRUPTION; ASTROCYTES; NETWORKS; STROKE;

D O I：

10.1073/pnas.1018790108

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Systemic delivery of bioactive molecules in the CNS is hampered by the blood-brain barrier, which has bottlenecked noninvasive physiological study of the brain and the development of CNS drugs. Here we report that irradiation with an ultrashort pulsed laser to the blood vessel wall induces transient leakage of blood plasma without compromising vascular integrity. By combining this method with a systemic injection, we delivered target molecules in various tissues, including the brain cortex. This tool allows minimally invasive local delivery of chemical probes, nanoparticles, and viral vectors into the brain cortex. Furthermore, we demonstrated astrocyte-mediated vasodilation in vivo without opening the skull, using this method to load a calcium indicator in conjunction with label-free photoactivation of astrocytes.

引用

页码：9256 / 9261

页数：6

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