Transfer functions linking neural calcium to single voxel functional ultrasound signal

被引：51

作者：

Aydin, Ali-Kemal ^{[1
,2
]}

Haselden, William D. ^{[3
,4
]}

Houssen, Yannick Goulam ^{[1
]}

Pouzat, Christophe ^{[5
]}

Rungta, Ravi L. ^{[1
,2
]}

Demene, Charlie ^{[6
]}

Tanter, Mickael ^{[6
]}

Drew, Patrick J. ^{[7
]}

Charpak, Serge ^{[1
,2
]}

Boido, Davide ^{[1
,8
]}

机构：

[1] Univ Paris, Lab Neurophysiol & New Microscopy, INSERM, U1128, Paris, France

[2] Sorbonne Univ, Inst Vis, CNRS, INSERM, Paris, France

[3] Penn State Univ, Med Scientist Training Program, University Pk, PA 16802 USA

[4] Penn State Univ, Neurosci Grad Program, University Pk, PA 16802 USA

[5] CNRS, UMR 8145, MAP5, Paris, France

[6] PSL Res Univ, CNRS, INSERM, Phys Med,ESPCI, Paris, France

[7] Penn State Univ, Dept Engn Sci & Mech, 227 Hammond Bldg, University Pk, PA 16802 USA

[8] Commissariat Energie Atom Saclay Ctr, NeuroSpin, Bat 145, F-91191 Gif Sur Yvette, France

来源：

NATURE COMMUNICATIONS | 2020年 / 11卷 / 01期

基金：

欧洲研究理事会;

关键词：

HEMODYNAMIC-RESPONSE; OLFACTORY-BULB; BLOOD-FLOW; DISCRIMINATION; STIMULATION; ACCURACY; SPEED; BOLD; TIME;

D O I：

10.1038/s41467-020-16774-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Functional ultrasound imaging (fUS) is an emerging technique that detects changes of cerebral blood volume triggered by brain activation. Here, we investigate the extent to which fUS faithfully reports local neuronal activation by combining fUS and two-photon microscopy (2PM) in a co-registered single voxel brain volume. Using a machine-learning approach, we compute and validate transfer functions between dendritic calcium signals of specific neurons and vascular signals measured at both microscopic (2PM) and mesoscopic (fUS) levels. We find that transfer functions are robust across a wide range of stimulation paradigms and animals, and reveal a second vascular component of neurovascular coupling upon very strong stimulation. We propose that transfer functions can be considered as reliable quantitative reporters to follow neurovascular coupling dynamics. Neurovascular coupling refers to changes in cerebral blood flow in response to neuronal stimulation, but to what extent this change can report neuronal activation is not known. Here the authors develop transfer functions between neural calcium signals and functional ultrasound changes in blood volume in co-registered single voxel brain volumes.

引用

页数：10

共 44 条

[1] Maintaining accuracy at the expense of speed: Stimulus similarity defines odor discrimination time in mice [J].