P2X7 receptor is essential for cross-dressing of bone marrow-derived dendritic cells

被引：11

作者：

Barrera-Avalos, Carlos ^{[1
,2
]}

Briceno, Pedro ^{[3
]}

Valdes, Daniel ^{[1
]}

Imarai, Monica ^{[1
,2
]}

Leiva-Salcedo, Elias ^{[1
]}

Rojo, Leonel E. ^{[1
,2
]}

Milla, Luis A. ^{[4
]}

Huidobro-Toro, Juan Pablo ^{[1
]}

Robles-Planells, Claudia ^{[1
,2
]}

Escobar, Alejandro ^{[5
]}

Di Virgilio, Francesco ^{[6
]}

Moron, Gabriel ^{[7
,8
]}

Sauma, Daniela ^{[3
]}

Acuna-Castillo, Claudio ^{[1
,2
]}

机构：

[1] Univ Santiago Chile, USACH, Fac Quim & Biol, Dept Biol, Santiago 3363, Chile

[2] Univ Santiago Chile, Ctr Biotecnol Acuicola, USACH, Santiago 3363, Chile

[3] Univ Chile, Fac Ciencias, Dept Biol, Santiago, Chile

[4] Univ Santiago Chile, Fac Ciencias Med, Escuela Med, Ctr Invest Biomed & Aplicadas, Santiago, Chile

[5] Univ Chile, Lab Biol Celular & Mol, Inst Invest Ciencias Odontol, Fac Odontol, Santiago, Chile

[6] Univ Ferrara, Dept Med Sci, Ferrara, Italy

[7] Univ Nacl Cordoba, Fac Ciencias Quim, Dept Bioquim Clin, Cordoba, Argentina

[8] Consejo Nacl Invest Cient & Tecn, Ctr Invest Bioquim Clin & Inmunol, Cordoba, Argentina

来源：

ISCIENCE | 2021年 / 24卷 / 12期

关键词：

MYCOBACTERIUM-TUBERCULOSIS; P2X(7) RECEPTOR; ATP; RELEASE; IDENTIFICATION; POLYMORPHISM; LYMPHOCYTES; ACTIVATION; EXOSOMES; ABSENCE;

D O I：

10.1016/j.isci.2021.103520

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

T cell activation requires the processing and presentation of antigenic peptides in the context of a major histocompatibility complex (MHC complex). Cross -dressing is a non-conventional antigen presentation mechanism, involving the transfer of preformed peptide/MHC complexes from whole cells, such as apoptotic cells (ACs) to the cell membrane of professional antigen-presenting cells (APCs), such as dendritic cells (DCs). This is an essential mechanism for the induction of immune response against viral antigens, tumors, and graft rejection, which until now has not been clarified. Here we show for first time that the P2X7 receptor (P2X7R) is crucial to induce cross-dressing between ACs and Bone-Marrow DCs (BMDCs). In controlled ex vivo assays, we found that the P2X7R in both ACs and BMDCs is required to induce membrane and fully functional peptide/MHC complex transfer to BMDCs. These findings show that acquisition of ACs-derived preformed antigen/MHC-I complexes by BMDCs requires P2X7R expression.

引用

页数：19

共 53 条

[1] Accelerated Tumor Progression in Mice Lacking the ATP Receptor P2X7 [J].