Collection of mutants for functional genomics in the legume Medicago truncatula

被引：3

作者：

Calderini, O. ^{[1
]}

Carelli, M. ^{[2
]}

Panara, F. ^{[1
]}

Biazzi, E. ^{[2
]}

Scotti, C. ^{[2
]}

Tava, A. ^{[2
]}

Porceddu, A. ^{[3
]}

Arcioni, S. ^{[1
]}

机构：

[1] CNR Ist Genet Vegetale, Perugia, Italy

[2] CRA Ctr Prod Foraggere & Lattiero Caseari, Lodi, Italy

[3] Univ Sassari, Dipartimento Sci Agron & Genet Vegetale Agr, I-07100 Sassari, Italy

来源：

PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION | 2011年 / 9卷 / 02期

关键词：

Medicago truncatula; mutants; Transposon tagging; TILLING; RETROTRANSPOSON; ARABIDOPSIS; MUTAGENESIS; EXPRESSION;

D O I：

10.1017/S1479262111000165

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We have established mutant collections of the model species Medicago truncatula according to current protocols. In particular, we used a transposon (Tnt1) tagging method and an ethyl methanesulfonate (EMS) mutagenesis approach (TILLING). The collections were subjected to both forward and reverse genetics screenings, and several mutants were isolated that affect plant traits (e. g. shoot, root developments, flower morphology, etc.) and also biosynthetic pathways of secondary compounds (saponins and tannins). Genes responsible for some of the mutations were cloned and further characterized.

引用

页码：174 / 176

页数：3

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