Olfactory ensheathing cells promote proliferation and inhibit neuronal differentiation of neural progenitor cells through activation of notch signaling

被引：34

作者：

Zhang, J. ^{[1
,2
]}

Wang, B. ^{[1
]}

Xiao, Z. ^{[1
]}

Zhao, Y. ^{[1
]}

Chen, B. ^{[1
]}

Han, J. ^{[1
]}

Gao, Y. ^{[1
]}

Ding, W. ^{[1
]}

Zhang, H. ^{[3
,4
]}

Dai, J. ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Mol Dev Biol, Beijing 100080, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China

[3] Chinese Acad Med Sci, Inst Basic Med Sci, Natl Lab Med Mol Biol, Dept Physiol & Pathophysiol, Beijing 100005, Peoples R China

[4] Peking Union Med Coll, Beijing 100005, Peoples R China

来源：

NEUROSCIENCE | 2008年 / 153卷 / 02期

基金：

中国国家自然科学基金;

关键词：

conditioned medium; spinal cord injury; DAPT;

D O I：

10.1016/j.neuroscience.2008.02.067

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A population of neural progenitor cells (NPCs) has been known to exist in adult spinal cord and migrate toward the lesion regions during spinal cord injury (SCI). Although there are some positive effects of the transplanted olfactory ensheathing cells (OECs) on axonal regeneration in SCI, little is known about the effects and the underlying mechanism of these grafted OECs on NPCs. In this study, we have investigated how soluble factors derived from rat OECs regulate the proliferation and differentiation of rat NPCs. The conditioned medium from cultured OECs showed its ability to promote proliferation and inhibit neuronal differentiation of NPCs. Notch signaling was apparently involved in this process. With the addition of DAPT, which inhibited Notch signaling, the effects of OEC-conditioned medium on NPCs were blocked. We thus conclude that diffusible factors released from OECs activate the Notch signaling pathway to stimulate the proliferation and suppress neuronal differentiation of NPCs. These findings reveal the likely limitation of using OECs transplantation for SCI repair. (c) 2008 IBRO. Published by Elsevier Ltd. All rights reserved.

引用

页码：406 / 413

页数：8

共 53 条

[11] rax, hes1, and notch1 promote the formation of Muller glia by postnatal retinal progenitor cells [J].