Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis)

被引：36
|
作者：
Mekuria, G
Ramesh, SA
Alberts, E
Bertozzi, T
Wirthensohn, M
Collins, G
Sedgley, M
机构：
[1] Univ Adelaide, Sch Agr & Wine, Glen Osmond, SA 5064, Australia
[2] S Australian Res & Dev Inst, Adelaide, SA 5064, Australia
来源：
JOURNAL OF VIROLOGICAL METHODS | 2003年 / 114卷 / 01期
关键词：
ilarvirus; Prunus necrotic ringspot virus; prune dwarf virus; ELISA; RT-PCR;
D O I：
10.1016/j.jviromet.2003.08.014
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A technique based on the reverse transcriptase-polymerase chain reaction (RT-PCR) has been developed to detect the presence of Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) simultaneously in almond. This paper presents the results of a 3-year study comparing both enzyme-linked immunosorbent assay (ELISA) and RT-PCR for the detection of PNRSV and PDV using 175 almond leaf samples. Multiplex RT-PCR was found to be more sensitive than ELISA, especially when followed by nested PCR for the detection of PDV. The RT-PCR technique has the added advantage that plant material can be tested at any time throughout the growing season. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码：65 / 69
页数：5
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