Analysis of biomarkers characteristic of porcine liver injury-from biomolecular logic gates to an animal model

被引：39

作者：

Halamkova, Lenka ^{[1
,2
]}

Halamek, Jan ^{[1
]}

Bocharova, Vera ^{[1
]}

Wolf, Steven ^{[1
]}

Mulier, Kristine E. ^{[3
]}

Beilman, Greg ^{[3
]}

Wang, Joseph ^{[4
]}

Katz, Evgeny ^{[1
]}

机构：

[1] Clarkson Univ, Dept Chem & Biomol Sci, Potsdam, NY 13699 USA

[2] Clarkson Univ, Dept Biol, Potsdam, NY 13699 USA

[3] Univ Minnesota, Minneapolis, MN 55455 USA

[4] Univ Calif San Diego, Dept NanoEngn, La Jolla, CA 92093 USA

来源：

ANALYST | 2012年 / 137卷 / 08期

基金：

美国国家科学基金会;

关键词：

BIOMEDICAL APPLICATIONS; MOLECULAR LOGIC; BIOSENSORS; SYSTEMS; SIGNALS; CURVES;

D O I：

10.1039/c2an00014h

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A biocatalytic cascade for the analysis of the simultaneous increase in the concentration of two biomarkers characteristic of liver injury (alanine transaminase, ALT, and lactate dehydrogenase, LDH) was tested on real samples acquired from an animal model (domestic pigs, Sus scrofa domesticus) suffering from traumatic liver injury. A two-step reaction biocatalyzed in the presence of both enzyme-biomarkers resulted in the oxidation of NADH followed by optical absorbance measurements. A simple qualitative, YES/NO, test allowed for distinction between animals with and without the presence of liver injury with the probability of 92%. These data represent the first demonstration of applying binary logic systems for the analysis of real biomedical samples.

引用

页码：1768 / 1770

页数：3

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