Influence of Glycosylation on Diagnostic and Prognostic Accuracy of N-Terminal Pro-B-Type Natriuretic Peptide in Acute Dyspnea: Data from the Akershus Cardiac Examination 2 Study

被引：46

作者：

Rosjo, Helge ^{[1
,2
,3
]}

Dahl, Mai Britt ^{[4
,5
]}

Jorgensen, Marit ^{[1
,2
,3
,4
,5
]}

Roysland, Ragnhild ^{[1
,2
,3
]}

Brynildsen, Jon ^{[1
,2
,3
]}

Cataliotti, Alessandro ^{[6
,7
,8
]}

Christensen, Geir ^{[6
,7
,8
]}

Hoiseth, Arne Didrik ^{[1
,2
,3
]}

Hagve, Tor-Arne ^{[9
,10
]}

Omland, Torbjorn ^{[1
,2
,3
]}

机构：

[1] Akershus Univ Hosp, Div Med, N-1478 Lorenskog, Norway

[2] Univ Oslo, Inst Clin Med, Ctr Heart Failure Res, Oslo, Norway

[3] Univ Oslo, Inst Clin Med, KG Jebsen Cardiac Res Ctr, Oslo, Norway

[4] Akershus Univ Hosp, Dept Clin Mol Biol, Lorenskog, Norway

[5] Univ Oslo, Inst Clin Med, Oslo, Norway

[6] Oslo Univ Hosp, Expt Med Res Inst, Oslo, Norway

[7] Univ Oslo, Inst Clin Med, Ctr Heart Failure Res, Oslo, Norway

[8] Univ Oslo, Inst Clin Med, KG Jebsen Cardiac Res Ctr, Oslo, Norway

[9] Akershus Univ Hosp, Div Diagnost & Technol, Sect Med Biochem, Lorenskog, Norway

[10] Univ Oslo, Inst Clin Med, Oslo, Norway

来源：

CLINICAL CHEMISTRY | 2015年 / 61卷 / 08期

关键词：

EMERGENCY-DEPARTMENT PRIDE; CONGESTIVE-HEART-FAILURE; EJECTION FRACTION; BNP; PRECURSOR; UTILITY; PLASMA; ASSAY;

D O I：

10.1373/clinchem.2015.239673

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

BACKGROUND: The N-terminal part of pro-B-type natriuretic peptide (NT-proBNP) is glycosylated, but whether glycosylation influences the diagnostic and prognostic accuracy of NT-proBNP measurements is not known. METHODS: We measured NT-proBNP concentrations of 309 patients with acute dyspnea by use of standard EDTA tubes and EDTA tubes pretreated with deglycosylation enzymes. The primary cause of dyspnea was classified as heart failure (HF) or non-HF, and the diagnosis was adjudicated by 2 independent physicians. We collected information on all-cause mortality during follow-up. RESULTS: In all, 142 patients (46%) were diagnosed with HF. NT-proBNP concentrations in nondeglycosylated samples distinguished HF patients from patients with non-HF related dyspnea [median 3588 (quartiles 1-3 1578-8404) vs 360 (126-1139) ng/L, P < 0.001], but concentrations were markedly higher in samples pretreated with deglycosylation enzymes (total NT-proBNP) [7497 (3374-14915) vs 798 (332-2296) ng/L, P < 0.001]. The AUC to separate HF patients from patients with non-HF related dyspnea was 0.871 (95% CI 0.829-0.907) for total NT-proBNP compared with 0.852 (0.807-0.890) for NT-proBNP measurements in standard EDTA plasma. During a median follow-up of 816 days, 112 patients (36%) died. Both NT-proBNP and total NT-proBNP concentrations were associated with mortality in separate multivariate models, but only total NT-proBNP concentrations provided added value to the basic risk model of our dataset as assessed by the net reclassification index: 0.24 (95% CI 0.003-0.384). There was a graded increase in risk across total NT-proBNP quartiles, in contrast with the results for NT-proBNP measurements. CONCLUSIONS: NT-proBNP concentrations were higher, and diagnostic and prognostic accuracy was improved, by pretreating tubes with deglycosylation enzymes. (C) 2015 American Association for Clinical Chemistry

引用

页码：1087 / 1097

页数：11

共 26 条

[11] ESC Guidelines for the diagnosis and treatment of acute and chronic heart failure 2012 The Task Force for the Diagnosis and Treatment of Acute and Chronic Heart Failure 2012 of the European Society of Cardiology. Developed in collaboration with the Heart Failure Association (HFA) of the ESC [J].

McMurray, John J. V. ;

Adamopoulos, Stamatis ;

Anker, Stefan D. ;

Auricchio, Angelo ;

Boehm, Michael ;

Dickstein, Kenneth ;

Falk, Volkmar ;

Filippatos, Gerasimos ;

Fonseca, Candida ;

Angel Gomez-Sanchez, Miguel ;

Jaarsma, Tiny ;

Kober, Lars ;

Lip, Gregory Y. H. ;

Maggioni, Aldo Pietro ;

Parkhomenko, Alexander ;

Pieske, Burkert M. ;

Popescu, Bogdan A. ;

Ronnevik, Per K. ;

Rutten, Frans H. ;

Schwitter, Juerg ;

Seferovic, Petar ;

Stepinska, Janina ;

Trindade, Pedro T. ;

Voors, Adriaan A. ;

Zannad, Faiez ;

Zeiher, Andreas ;

Bax, Jeroen J. ;

Baumgartner, Helmut ;

Ceconi, Claudio ;

Dean, Veronica ;

Deaton, Christi ;

Fagard, Robert ;

Funck-Brentano, Christian ;

Hasdai, David ;

Hoes, Arno ;

Kirchhof, Paulus ;

Knuuti, Juhani ;

Kolh, Philippe ;

McDonagh, Theresa ;

Moulin, Cyril ;

Popescu, Bogdan A. ;

Reiner, Zeljko ;

Sechtem, Udo ;

Sirnes, Per Anton ;

Tendera, Michal ;

Torbicki, Adam ;

Vahanian, Alec ;

Windecker, Stephan ;

McDonagh, Theresa ;

Sechtem, Udo .

EUROPEAN HEART JOURNAL, 2012, 33 (14) :1787-1847

[12] N-terminal pro-B-type natriuretic peptide testing improves the management of patients with suspected acute heart failure [J].

Moe, Gordon W. ;

Howlett, Jonathan ;

Januzzi, James L. ;

Zowall, Hanna .

CIRCULATION, 2007, 115 (24) :3103-3110

[13] Direct Immunochemiluminescent Assay for proBNP and Total BNP in Human Plasma proBNP and Total BNP Levels in Normal and Heart Failure [J].

Nishikimi, Toshio ;

Okamoto, Hiroyuki ;

Nakamura, Masahiro ;

Ogawa, Naoko ;

Horii, Kazukiyo ;

Nagata, Kiyoshi ;

Nakagawa, Yasuaki ;

Kinoshita, Hideyuki ;

Yamada, Chinatsu ;

Nakao, Kazuhiro ;

Minami, Takeya ;

Kuwabara, Yoshihiro ;

Kuwahara, Koichiro ;

Masuda, Izuru ;

Kangawa, Kenji ;

Minamino, Naoto ;

Nakao, Kazuwa .

PLOS ONE, 2013, 8 (01)

[14] The effect of glycosylation on plasma N-terminal proBNP-76 levels in patients with heart or renal failure [J].

Nishikimi, Toshio ;

Ikeda, Masashi ;

Takeda, Yosuke ;

Ishimitsu, Toshihiko ;

Shibasaki, Ikuko ;

Fukuda, Hirotsugu ;

Kinoshita, Hideyuki ;

Nakagawa, Yasuaki ;

Kuwahara, Koichiro ;

Nakao, Kazuwa .

HEART, 2012, 98 (02) :152-161

[15] The effects of ejection fraction on N-terminal ProBNP and BNP levels in patients with acute CHF: Analysis from the ProBNP Investigation of Dyspnea in the Emergency Department (PRIDE) study [J].

O'Donoghue, M ;

Chen, A ;

Baggish, AL ;

Anwaruddin, S ;

Krauser, DG ;

Tung, R ;

Januzzi, JL .

JOURNAL OF CARDIAC FAILURE, 2005, 11 (05) :S9-S14

[16]

Omland Torbjorn, 2008, Crit Care Med, V36, pS17, DOI 10.1097/01.CCM.0000296266.74913.85

[17] Diagnostic utility of a single-epitope sandwich B-type natriuretic peptide assay in stable coronary artery disease: Data from the Akershus Cardiac Examination (ACE) 1 Study [J].

Rosjo, Helge ;

Tamm, Natalia N. ;

Kravdal, Gunnhild ;

Seferian, Karina R. ;

Hoiseth, Arne Didrik ;

Nygard, Stale ;

Badr, Pirouz ;

Roysland, Ragnhild ;

Omland, Torbjorn .

CLINICAL BIOCHEMISTRY, 2012, 45 (16-17) :1269-1275

[18] The precursor to B-type natriuretic peptide is an O-linked glycoprotein [J].

Schellenberger, Ute ;

O'Rear, Jessica ;

Guzzetta, Andrew ;

Jue, Rodney A. ;

Protter, Andrew A. ;

Pollitt, N. Stephen .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2006, 451 (02) :160-166

[19] Immunodetection of glycosylated NT-proBNP circulating in human blood [J].

Seferian, Karina R. ;

Tamm, Natalia N. ;

Semenov, Alexander G. ;

Tolstaya, Anastasia A. ;

Koshkina, Ekaterina V. ;

Krasnoselsky, Mihail I. ;

Postnikov, Alexander B. ;

Serebryanaya, Daria V. ;

Apple, Fred S. ;

Murakarni, MaryAnn M. ;

Katrukha, Alexey G. .

CLINICAL CHEMISTRY, 2008, 54 (05) :866-873

[20] The brain natriuretic peptide (BNP) precursor is the major immunoreactive form of BNP in patients with heart failure [J].

Seferian, Karina R. ;

Tamm, Natalia N. ;

Semenov, Alexander G. ;

Mukharyamova, Kadriya S. ;

Tolstaya, Anastasya A. ;

Koshkina, Ekaterina V. ;

Kara, Andrei N. ;

Krasnoselsky, Mihail I. ;

Apple, Fred S. ;

Esakova, Tatiana V. ;

Filatov, Vladimir L. ;

Katrukha, Alexey G. .

CLINICAL CHEMISTRY, 2007, 53 (05) :866-873

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