Postmortem Changes in Pork Muscle Protein Phosphorylation in Relation to the RN Genotype

被引：28

作者：

Lametsch, Rene ^{[1
]}

Larsen, Martin R. ^{[2
]}

Essen-Gustavsson, Birgitta ^{[4
]}

Jensen-Waern, Marianne ^{[4
]}

Lundstrom, Kerstin ^{[3
]}

Lindahl, Gunilla ^{[1
,3
]}

机构：

[1] Univ Copenhagen, Dept Food Sci, DK-1958 Frederiksberg C, Denmark

[2] Univ So Denmark, Dept Biochem & Mol Biol, DK-5230 Odense, Denmark

[3] Swedish Univ Agr Sci, Dept Food Sci, SE-75007 Uppsala, Sweden

[4] Swedish Univ Agr Sci, Dept Clin Sci, SE-75007 Uppsala, Sweden

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2011年 / 59卷 / 21期

关键词：

Protein phosphorylation; pork; RN(-) genotype; postmortem metabolism; glycolysis; glycogenolysis; REGULATORY LIGHT-CHAIN; SKELETAL-MUSCLE; LONGISSIMUS MUSCLE; ENERGY-METABOLISM; KINASE; GLYCOLYSIS; CONTRACTION; GLYCOGEN; PIGS; MECHANISMS;

D O I：

10.1021/jf201936h

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Postmortem changes in pork muscle protein phosphorylation in relation to the RN(-) genotype were investigated using one-dimensional gel electrophoresis and a phosphor specific staining The phosphorylation levels of several protein bands were found to be affected by the RN(-) genotype and to change during postmortem development Glycogen phosphorylase, phosphofructokinase, and pyruvate kinase were found in protein bands affected by the RN(-) genotype, and the phosphorylation profile indicates that part of the increased rate and extended pH decline of the RN(-) genotype could lie a consequence of phosphorylation of these key enzymes during the postmortem metabolism The results illustrate that the protein phosphorylation level of the muscle proteins could be interpreted as a global metabolic fingerprint containing information about the activity status of the enzymes in the postmortem metabolism

引用

页码：11608 / 11615

页数：8

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