Overexpression of inflammatory-related and nitric oxide synthase genes in olfactory bulbs from frontal lobe epilepsy patients

被引:22
作者
Fabian Mercado-Gomez, Octavio [1 ]
Cordova-Davalos, Laura [1 ,2 ,3 ]
Garcia-Betanzo, Delfina [1 ]
Rocha, Luisa [2 ]
Arturo Alonso-Vanegas, Mario [3 ]
Cienfuegos, Jesus [3 ]
Guevara-Guzman, Rosalinda [1 ]
机构
[1] Univ Nacl Autonoma Mexico, Fac Med, Dept Fisiol, Mexico City 04510, DF, Mexico
[2] Ctr Invest & Estudios Avanzados, Dept Farmacobiol, Mexico City 14330, DF, Mexico
[3] Inst Nacl Neurol & Neurocirugia Manuel Velasco Su, Secc Neurocirugia, Mexico City 14269, DF, Mexico
关键词
Frontal epilepsy; Olfactory bulb; Olfactoy disfunction; Hipoocampus; NOS; TEMPORAL-LOBE;
D O I
10.1016/j.eplepsyres.2018.09.012
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Neuroinflammation has been shown to constitute a crucial mechanism in the pathophysiology of epileptic brain and several genes of inflammatory mediators have been detected in surgically resected hippocampus tissue but not in non-related seizure brain regions. Interestingly, it has been reported an olfactory dysfunction in frontal lobe epilepsy (FLE). Our aim was to quantify the gene expression of inflammatory-related and nitric oxide synthase genes in olfactory bulbs (OB) tissue from FLE patients. RNA was isolated from OB resection of FLE patients and autopsy subjects without any neurological disease (n = 7, each). After cDNA synthesis, we performed qPCR for interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), nuclear factor kappa B p65 (RELA), Toll-like receptor 4 (TLR 4), its agonist high mobility group box 1 (HMGB 1) as well nitric oxide synthase isozymes (NOS 1, 2 and 3). We found a significant increase in gene expression of pro-inflammatory cytokines (IL-1 beta, IL-6 and TNF alpha), TLR4 receptor and in its agonist HMGB1 and the downstream transcription factor NF kappa B p65. Moreover, we observed an increase of both NOS1 and NOS3 and a slightly increase of NOS2; however, it was not significant. Our study describes the overexpression of inflammatory-related genes and NOS isozymes in OB from FLE patients. Even though, the number of patients was limited, our findings could point out that neuroinflammation and nitrosative stress-related genes in the OB could be produced in general manner in all brain regions and thus contribute in part, to the olfactory dysfunction observed in FLE patients.
引用
收藏
页码:37 / 43
页数:7
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