Extract of Moutan radicis cortex and Cinnamomi ramulus ameliorates laser-induced choroidal neovascularization in Brown-Norway rats

被引：3

作者：

Lee, Ik Soo ^{[1
]}

Kim, Chan-Sik ^{[2
]}

Kim, Junghyun ^{[3
]}

Jo, Kyuhyung ^{[1
]}

Hyun, Soo Wang ^{[1
,4
]}

Zhang, Haiying ^{[5
]}

Noh, MinYoung ^{[6
]}

Kwon, Young-Guen ^{[5
,6
]}

Kim, MyungHwa ^{[5
]}

Kim, Jin Sook ^{[1
]}

机构：

[1] Korea Inst Oriental Med, Herbal Med Res Div, Daejeon 34054, South Korea

[2] Korea Inst Oriental Med, Clin Med Div, Daejeon 34054, South Korea

[3] Jeonbuk Natl Univ, Sch Dent, Dept Oral Pathol, Jeonju 54896, South Korea

[4] Honam Natl Inst Biol Resources, Pract Res Div, Mokpo 58762, South Korea

[5] Curacle Co Ltd, Seongnam 13449, South Korea

[6] Yonsei Univ, Coll Life Sci & Biotechnol, Dept Biochem, Seoul 120749, South Korea

来源：

PHYTOMEDICINE | 2022年 / 94卷

关键词：

Anti-angiogenic action; Choroidal neovascularization; 50% Ethanol extract of Moutan radicis cortex and Cinnamomi ramulus; GENE-EXPRESSION; CELL-MIGRATION; ACTIVATION; ACID;

D O I：

10.1016/j.phymed.2021.153794

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Background: Moutan radicis cortex (MRC) and Cinnamomi ramulus (CR) are commonly used in eastern Asian traditional medicine to treat various diseases including cerebrovascular and cardiovascular, and have wide spectrum of pharmacological activities. However, the effect against laser-induced choroidal neovascularization (CNV) of extract of MRC and CR (1:1) (MRCCR) has not yet been studied. Purpose: Our aim was to investigate the inhibitory effect of MRCCR on pathological CNV in laser-treated Brown Norway (BN) rats. Methods: MRCCR (60, 90 mg/kg) was orally administered twice per day for 15 days from the day of CNV formation in laser-treated BN rats. Effects of MRCCR or its constituents on cell migration, tube formation, hyper permeability and phosphorylation of FAK/p38 MAPK were confirmed in humane retinal microvascular endothelial cells or human retinal pigment epithelial cells. Results: MRCCR significantly reduced the CNV lesions areas and the extent of fluorescein leakage. MRCCR and its constituents such as ellagic acid, paeonol or gallic acid decreased cell migration, tube formation or hyper permeability. MRCCR inhibited the phosphorylation of FAK and p38 MAPK. Conclusion: Combining the oral MRCCR and intravitreal injection of anti-VEGF medicine may result in a more potent therapeutic effect and consequently bring the reduction in eye injection numbers for patients with wet AMD.

引用

页数：8

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