Increased integrity of circulating cell-free DNA in plasma of patients with acute leukemia

被引：61

作者：

Gao, Yu-Jie ^{[1
]}

He, Yu-Juan ^{[1
]}

Yang, Zai-Lin ^{[2
]}

Shao, Hui-Yuan ^{[1
]}

Zuo, Yan ^{[1
]}

Bai, Yao ^{[3
]}

Chen, Hui ^{[3
]}

Chen, Xian-Chun ^{[1
]}

Qin, Feng-Xian ^{[1
]}

Tan, Shi ^{[1
]}

Wang, Jian ^{[1
]}

Wang, Li ^{[4
]}

Zhang, Ling ^{[1
]}

机构：

[1] Chongqing Med Univ, Fac Lab Med, Key Lab Lab Med Diagnost, Minist Educ, Chongqing 400016, Peoples R China

[2] Third Mil Med Univ, SW Hosp, Ctr Hematol, Chongqing, Peoples R China

[3] Chongqing Med Univ, Affiliated Hosp 1, Dept Lab Med, Chongqing 400016, Peoples R China

[4] Chongqing Med Univ, Affiliated Hosp 1, Dept Hematol, Chongqing 400016, Peoples R China

来源：

CLINICAL CHEMISTRY AND LABORATORY MEDICINE | 2010年 / 48卷 / 11期

基金：

美国国家科学基金会;

关键词：

acute leukemia; cell-free DNA; DNA; integrity; plasma; MINIMAL RESIDUAL DISEASE; PERIPHERAL-BLOOD PLASMA; ACUTE MYELOID-LEUKEMIA; CANCER-PATIENTS; QUANTITATIVE PCR; QUANTIFICATION; SERUM; DIAGNOSIS; MARKER;

D O I：

10.1515/CCLM.2010.311

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: Increased cell-free DNA (cf-DNA) and the integrity of cf-DNA in plasma of patients with cancer has been described. We investigated the clinical utility of cf-DNA in the detection and monitoring of progression of leukemia. Methods: Plasma samples from 60 patients with acute leukemia were analyzed in comparison to plasma from 30 healthy controls. Plasma DNA was determined by quantitative PCR (qPCR) by amplifying the beta-actin gene (ACTB). The DNA integrity index was calculated as the ratio of qPCR results (ACTB384/106). Paired diagnostic/complete remission (CR)/relapse samples from eight of 60 patients were analyzed, and the minimum residual disease (MRD) situations were monitored. Results: DNA concentrations (median: 8.80 ng/mL, p=0.004) and DNA integrity (median: 0.51, p<0.001) in cancer patients were significantly higher. Receiver operating characteristic (ROC) curve analysis showed that the area under the ROC curve of DNA and DNA integrity were 0.79 and 0.88, respectively. DNA integrity at CR had a distinct reduction and then an increase at relapse. DNA integrity in CR cases was higher than that observed in healthy controls. Conclusions: Our preliminary data suggest that plasma DNA integrity is increased in acute leukemia and may be a potential biomarker for monitoring MRD. However, more work is needed. Clin Chem Lab Med 2010;48:1651-6.

引用

页码：1651 / 1656

页数：6

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