Expression of the chemokine receptor CXCR4 in human hepatocellular carcinoma and its role in portal vein tumor thrombus

被引：29

作者：

Li, Nan ^{[1
]}

Guo, Weixing ^{[1
]}

Shi, Jie ^{[1
]}

Xue, Jie ^{[1
]}

Hu, Huasheng ^{[1
]}

Xie, Dong ^{[2
]}

Wu, Mengchao ^{[1
]}

Cheng, Shuqun ^{[1
]}

机构：

[1] Second Mil Med Univ, Dept Oncol Comprehens Treatment, Eastern Hepatobiliary Surg Hosp, Shanghai 200438, Peoples R China

[2] Chinese Acad Sci, Inst Nutr Sci, Shanghai 200032, Peoples R China

来源：

JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH | 2010年 / 29卷

关键词：

CELLS; CLONING;

D O I：

10.1186/1756-9966-29-156

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: This study was conducted to investigate the expression of CXCR4 in portal vein tumor thrombus (PVTT) tissue and its possible role in the invasiveness of tumor thrombus cells. Methods: We detected differential expression of CXCR4 between PVTT and hepatocellular carcinoma (HCC) by an immunohistochemical assay. Lentivirus-mediated RNA interference and a migration assay were performed on human primary cells derived from PVTT to study the impact of CXCR4 on the invasiveness of HCC. Results: The expression of CXCR4 in tumor thrombus tissue was higher than that in HCC tissue. The invasion ratio of PVTT cells was significantly decreased (P < 0.05) after being infected with a CXCR4-targeting siRNA lentivirus, indicating that downregulation of CXCR4 by lentivirus-mediated RNA interference significantly impaired the invasive potential of PVTT. Conclusions: These results indicate that CXCR4 is an effective curative target for hepatocellular carcinomas with PVTT.

引用

页数：8

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