Mixed-mode chromatography and its applications to biopolymers

被引：176

作者：

Yang, Yun ^{[1
]}

Geng, Xindu ^{[1
]}

机构：

[1] Northwest Univ, Inst Modern Separat Sci, Key Lab Synthet & Nat Funct Mol Chem, Minist Educ,Shaanxi Key Lab Modern Separat Sci, Xian 710069, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2011年 / 1218卷 / 49期

关键词：

Mixed-mode chromatography; Stationary phase; Molecular interactions; Stoichiometric displacement theory; Biopolymer; PERFORMANCE LIQUID-CHROMATOGRAPHY; INTERACTION/CATION-EXCHANGE CHROMATOGRAPHY; HYDROPHILIC-INTERACTION CHROMATOGRAPHY; REVERSED-PHASE; CAPILLARY ELECTROCHROMATOGRAPHY; ANION-EXCHANGE; ION-EXCHANGE; STATIONARY PHASES; MONOLITHIC COLUMNS; RETENTION BEHAVIOR;

D O I：

10.1016/j.chroma.2011.10.009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mixed-mode chromatography is a type of chromatography in which a chromatographic stationary phase interacts with solutes through more than one interaction mode. This technique has been growing rapidly because of its advantages over conventional chromatography, such as its high resolution, high selectivity, high sample loading, high speed, and the ability to replace two conventionally corresponding columns in certain circumstances. In this work, some aspects of the development of mixed-mode chromatography are reviewed, such as stationary phase preparation, combinations of various separation modes, separation mechanisms, typical applications to biopolymers and peptides, and future prospects. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：8813 / 8825

页数：13

共 161 条

[31] Temperature selectivity effects in reversed-phase liquid chromatography due to conformation differences between helical and non-helical peptides [J].