Thickness of Multiwalled Carbon Nanotubes Affects Their Lung Toxicity

被引：87

作者：

Fenoglio, Ivana ^{[1
,5
,6
]}

Aldieri, Elisabetta ^{[2
,5
]}

Gazzano, Elena ^{[2
,5
]}

Cesano, Federico ^{[1
,6
]}

Colonna, Massimiliano ^{[1
,5
]}

Scarano, Domenica ^{[1
,6
]}

Mazzucco, Gianna ^{[4
]}

Attanasio, Angelo ^{[4
]}

Yakoub, Yousof ^{[3
]}

Lison, Dominique ^{[3
]}

Fubini, Bice ^{[1
,5
,6
]}

机构：

[1] Univ Turin, Dept Chem, I-10125 Turin, Italy

[2] Univ Turin, Dipartimento Genet Biol & Biochim, I-10126 Turin, Italy

[3] Catholic Univ Louvain, Louvain Ctr Toxicol & Appl Pharmacol, B-1200 Brussels, Belgium

[4] Univ Turin, Dipartimento Sci Biomed & Oncol Umana, I-10126 Turin, Italy

[5] Univ Turin, Interdept Ctr G Scansetti Studies Asbestos & Tox, I-10125 Turin, Italy

[6] Univ Turin, Interdept Ctr Nanostruct Interfaces & Surfaces NI, I-10125 Turin, Italy

来源：

CHEMICAL RESEARCH IN TOXICOLOGY | 2012年 / 25卷 / 01期

关键词：

STRUCTURAL DEFECTS PLAY; PROTEIN ADSORPTION; MAJOR ROLE; PULMONARY; SINGLE; ASBESTOS; NANOPARTICLES; CYTOTOXICITY; INDUCTION; EXPOSURE;

D O I：

10.1021/tx200255h

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Two samples of highly pure multiwalled carbon nanotubes (MWCNTs) similar in hydrophobicity and surface reactivity were prepared with similar length, <5 mu m, but markedly different diameter (9.4 vs 70 nm). The samples were compared for their cytotoxic activity, uptake, and ability to induce oxidative stress (ROS production and intracellular GSH depletion) in vitro in murine alveolar macrophages (MH-S). The in vivo toxicity was evaluated by measuring biochemical (LDH activity and total proteins) and cellular responses in bronchoalveolar lavage (BAL) after intratracheal instillation in rats. Both samples were internalized in MH-S cells. However, thin MWCNTs appeared significantly more toxic than the thicker ones, both in vitro and in vivo, when compared on a mass-dose basis. The data reported herein suggest that the nanotube diameter is an important parameter to be considered in the toxicological assessment of CNTs.

引用

页码：74 / 82

页数：9

共 63 条

[1] Multi-walled carbon nanotubes induce T lymphocyte apoptosis [J].