Dynamic Changes in the MicroRNA Expression Profile Reveal Multiple Regulatory Mechanisms in the Spinal Nerve Ligation Model of Neuropathic Pain

被引:120
作者
von Schack, David [1 ]
Agostino, Michael J. [1 ]
Murray, B. Stuart [1 ]
Li, Yizheng [1 ]
Reddy, Padmalatha S. [1 ]
Chen, Jin [2 ]
Choe, Sung E. [1 ]
Strassle, Brian W. [2 ]
Li, Christine [1 ]
Bates, Brian [1 ]
Zhang, Lynn [1 ]
Hu, Huijuan [2 ]
Kotnis, Smita [2 ]
Bingham, Brendan [2 ]
Liu, Wei [1 ]
Whiteside, Garth T. [2 ]
Samad, Tarek A. [2 ]
Kennedy, Jeffrey D. [2 ]
Ajit, Seena K. [2 ,3 ]
机构
[1] Pfizer Global Res & Dev, Biol Technol, Cambridge, MA USA
[2] Pfizer Global Res & Dev, Neurosci Discovery, Princeton, NJ USA
[3] Drexel Univ, Dept Physiol & Pharmacol, Coll Med, Philadelphia, PA 19104 USA
关键词
DORSAL-ROOT GANGLION; TACTILE ALLODYNIA; PROTEIN-SYNTHESIS; P2X(4) RECEPTORS; GENE-EXPRESSION; UP-REGULATION; RAT; INJURY; IDENTIFICATION; SPECIFICITY;
D O I
10.1371/journal.pone.0017670
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Neuropathic pain resulting from nerve lesions or dysfunction represents one of the most challenging neurological diseases to treat. A better understanding of the molecular mechanisms responsible for causing these maladaptive responses can help develop novel therapeutic strategies and biomarkers for neuropathic pain. We performed a miRNA expression profiling study of dorsal root ganglion (DRG) tissue from rats four weeks post spinal nerve ligation (SNL), a model of neuropathic pain. TaqMan low density arrays identified 63 miRNAs whose level of expression was significantly altered following SNL surgery. Of these, 59 were downregulated and the ipsilateral L4 DRG, not the injured L5 DRG, showed the most significant downregulation suggesting that miRNA changes in the uninjured afferents may underlie the development and maintenance of neuropathic pain. TargetScan was used to predict mRNA targets for these miRNAs and it was found that the transcripts with multiple predicted target sites belong to neurologically important pathways. By employing different bioinformatic approaches we identified neurite remodeling as a significantly regulated biological pathway, and some of these predictions were confirmed by siRNA knockdown for genes that regulate neurite growth in differentiated Neuro2A cells. In vitro validation for predicted target sites in the 3'-UTR of voltage-gated sodium channel Scn11a, alpha 2/delta1 subunit of voltage-dependent Ca-channel, and purinergic receptor P2rx ligand-gated ion channel 4 using luciferase reporter assays showed that identified miRNAs modulated gene expression significantly. Our results suggest the potential for miRNAs to play a direct role in neuropathic pain.
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页数:14
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