Composition of anthocyanins in pomegranate flowers and their antioxidant activity

被引:81
作者
Zhang, Lihua [1 ,2 ]
Fu, Quanjuan [1 ]
Zhang, Yuanhu [1 ]
机构
[1] Shandong Agr Univ, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
[2] Zaozhuang Vocat Coll, Zaozhuang 277019, Peoples R China
关键词
Pomegranate flowers; Anthocyanins; Antioxidant activity; Identification; PERFORMANCE LIQUID-CHROMATOGRAPHY; PUNICA-GRANATUM L; EXTRACT; JUICE; PURIFICATION; PELARGONIDIN; GAMMA;
D O I
10.1016/j.foodchem.2011.01.077
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Anthocyanins in the flowers of pomegranate (Punica granatum L) were extracted and separated by reverse-phase high-performance liquid chromatography and Sephadex LH-20 chromatography. Two anthocyanins were identified to be pelargonidin 3,5-diglucoside and pelargonidin 3-glucoside by spectral methods, ultraviolet-visible spectroscopy, (1)H nuclear magnetic resonance spectroscopy, mass spectroscopy and comparisons with the literature. These two anthocyanins were identified from pomegranate flowers for the first time. Furthermore, the antioxidant activities of purified anthocyanins were screened for their antioxidative potential using 2,2'-diphenyl-1-picryl hydrazyl (DPPH) and 3-ethyl-benzo-thiazoline-6-sulphonate (ABTS) systems. The purified anthocyanins showed strong radical scavenging activities. Pelargonidin 3-glucoside showed higher antioxidant activity (50% inhibition values of 19.56 mu g/ml for DPPH and Trolox equivalents antioxidant activity of 2.39 mu g/mu g for ABTS) than pelargonidin 3,5-diglucoside. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:1444 / 1449
页数:6
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