Control of chrysanthemum flowering through integration with an aging pathway

被引：136

作者：

Wei, Qian ^{[1
]}

Ma, Chao ^{[1
]}

Xu, Yanjie ^{[1
]}

Wang, Tianle ^{[1
]}

Chen, Yiyu ^{[1
]}

Lu, Jing ^{[1
]}

Zhang, Lili ^{[1
]}

Jiang, Cai-Zhong ^{[2
,3
]}

Hong, Bo ^{[1
]}

Gao, Junping ^{[1
]}

机构：

[1] China Agr Univ, Dept Ornamental Hort, Beijing Key Lab Dev & Qual Control Ornamental Cro, Beijing 100193, Peoples R China

[2] ARS, Crops Pathol & Genet Res Unit, USDA, Davis, CA 95616 USA

[3] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA

来源：

NATURE COMMUNICATIONS | 2017年 / 8卷

基金：

中国国家自然科学基金;

关键词：

ABIOTIC STRESS TOLERANCE; BOX GENE SPL3; TRANSCRIPTION FACTORS; ARABIDOPSIS-THALIANA; FUNCTIONAL-ANALYSIS; PHASE-CHANGE; NF-Y; EXPRESSION; MICRORNA; BINDING;

D O I：

10.1038/s41467-017-00812-0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Age, as a threshold of floral competence acquisition, prevents precocious flowering when there is insufficient biomass, and ensures flowering independent of environmental conditions; however, the underlying regulatory mechanisms are largely unknown. In this study, silencing the expression of a nuclear factor gene, CmNF-YB8, from the short day plant chrysanthemum ( Chrysanthemum morifolium), results in precocious transition from juvenile to adult, as well as early flowering, regardless of day length conditions. The expression of SQUAMOSA PROMOTER BINDING-LIKE (SPL) family members, SPL3, SPL5, and SPL9, is upregulated in CmNFYB8-RNAi plants, while expression of the microRNA, cmo-MIR156, is downregulated. In addition, CmNF-YB8 is shown to bind to the promoter of the cmo-MIR156 gene. Ectopic expression of cmo-miR156, using a virus-based microRNA expression system, restores the early flowering phenotype caused by CmNF-YB8 silencing. These results show that CmNF-YB8 influences flowering time through directly regulating the expression of cmo-MIR156 in the aging pathway.

引用

页数：11

共 65 条

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