Optimization of a Method for the Simultaneous Extraction of Polar and Non-Polar Oxylipin Metabolites, DNA, RNA, Small RNA, and Protein from a Single Small Tissue Sample

被引:9
|
作者
Hasegawa, Yu [1 ]
Otoki, Yurika [1 ,2 ]
McClorry, Shannon [3 ]
Coates, Laurynne C. [1 ]
Lombardi, Rachel L. [1 ]
Taha, Ameer Y. [1 ]
Slupsky, Carolyn M. [1 ,3 ]
机构
[1] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA
[2] Tohoku Univ, Grad Sch Agr Sci, Sendai, Miyagi 9808572, Japan
[3] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA
基金
日本学术振兴会;
关键词
molecular biology; analytical chemistry; DNA; RNA; protein; metabolites; extraction; purification; tissue; systems biology;
D O I
10.3390/mps3030061
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A more comprehensive picture of tissue biology can be obtained through the application and integration of multiple omic technologies. However, the common challenge in working with a precious sample is having a sample too small to separately extract analytes of interest for each experiment. Considering the high heterogeneity that can be present in a single tissue sample, extracting all biomolecules from a single and undivided tissue is preferable because it allows direct comparison of results. Here, we combined a modified Folch extraction method with DNA, RNA, small RNA, and protein extraction using two commercial kits, which allowed us to extract polar metabolites and non-polar oxylipin metabolites, DNA, RNA, small RNA, and protein simultaneously from a small tissue sample. The method was validated in terms of quantity and quality of analytes for downstream analyses.
引用
收藏
页码:1 / 14
页数:14
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