共 5 条
Optimization of a Method for the Simultaneous Extraction of Polar and Non-Polar Oxylipin Metabolites, DNA, RNA, Small RNA, and Protein from a Single Small Tissue Sample
被引:9
|作者:
Hasegawa, Yu
[1
]
Otoki, Yurika
[1
,2
]
McClorry, Shannon
[3
]
Coates, Laurynne C.
[1
]
Lombardi, Rachel L.
[1
]
Taha, Ameer Y.
[1
]
Slupsky, Carolyn M.
[1
,3
]
机构:
[1] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA
[2] Tohoku Univ, Grad Sch Agr Sci, Sendai, Miyagi 9808572, Japan
[3] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA
基金:
日本学术振兴会;
关键词:
molecular biology;
analytical chemistry;
DNA;
RNA;
protein;
metabolites;
extraction;
purification;
tissue;
systems biology;
D O I:
10.3390/mps3030061
中图分类号:
Q5 [生物化学];
学科分类号:
071010 ;
081704 ;
摘要:
A more comprehensive picture of tissue biology can be obtained through the application and integration of multiple omic technologies. However, the common challenge in working with a precious sample is having a sample too small to separately extract analytes of interest for each experiment. Considering the high heterogeneity that can be present in a single tissue sample, extracting all biomolecules from a single and undivided tissue is preferable because it allows direct comparison of results. Here, we combined a modified Folch extraction method with DNA, RNA, small RNA, and protein extraction using two commercial kits, which allowed us to extract polar metabolites and non-polar oxylipin metabolites, DNA, RNA, small RNA, and protein simultaneously from a small tissue sample. The method was validated in terms of quantity and quality of analytes for downstream analyses.
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页码:1 / 14
页数:14
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