Applications of multiphoton polarization and generalized polarization microscopy in elucidating transdermal delivery pathways

被引：0

作者：

Yen, SA ^{[1
]}

Su, JW ^{[1
]}

Lo, W ^{[1
]}

Lin, SJ ^{[1
]}

Jee, SH ^{[1
]}

Dong, CY ^{[1
]}

机构：

[1] Natl Taiwan Univ, Dept Phys, Taipei 106, Taiwan

来源：

Photonic Therapeutics and Diagnostics | 2005年 / 5686卷

关键词：

multiphoton microscopy; polarization; generalized polarization; skin; transdermal delivery;

D O I：

10.1117/12.587426

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Physical and chemical enhancement techniques have proven to be useful in promoting transdermal delivery of therapeutic agents. However, it has been difficult to follow the changes to the skin during these processes microscopically. In this work, we use multiphoton polarization (P) and generalized polarization (GP) imaging of the membrane probe Laurdan to monitor changes to the stratum corneum under conditions of chemically enhanced delivery by oleic acid. We show that the combination of P and GP Laurdan imaging helps to reveal both the physical and chemical changes to the stratum corneum under enhanced delivery. Our work can help to develop more. efficient transdermal delivery strategies.

引用

页码：59 / 66

页数：8

共 10 条

[1] Two photon fluorescence microscopy of coexisting lipid domains in giant unilamellar vesicles of binary phospholipid mixtures [J].